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Literature summary for 2.3.2.15 extracted from

  • Olsson, S.; Penacho, V.; Puente-Sanchez, F.; Diaz, S.; Gonzalez-Pastor, J.E.; Aguilera, A.
    Horizontal gene transfer of phytochelatin synthases from bacteria to extremophilic green algae (2017), Microb. Ecol., 73, 50-60 .
    View publication on PubMed
Search for more literature for 2.3.2.15

Cloned(Commentary)

Cloned (Comment) Organism
gene CaPCS1, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotyping, recombinant expression in Escherichia coli improves the cadmium resistance of the bacteria, quantitative RT-PCR enzyme expression analysis Chlamydomonas acidophila
gene CaPCS2, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotyping, recombinant expression in Escherichia coli improves the cadmium resistance of the bacteria, quantitative RT-PCR enzyme expression analysis Chlamydomonas acidophila
gene DaPCS1, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotyping, recombinant expression in Escherichia coli, quantitative RT-PCR enzyme expression analysis Dunaliella acidophila

Organism

Organism UniProt Comment Textmining
Chlamydomonas acidophila
-
 collected from water samples taken in 2010 at the CEM station of Rio Tinto (SW Spain)
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Chlamydomonas acidophila RT46
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 collected from water samples taken in 2010 at the CEM station of Rio Tinto (SW Spain)
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Dunaliella acidophila
-
 collected from water samples taken in 2010 at the RICHI sampling station of Rio Tinto (SW Spain)
-
Dunaliella acidophila RT5
-
 collected from water samples taken in 2010 at the RICHI sampling station of Rio Tinto (SW Spain)
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information the cells are grown in ARTII medium Dunaliella acidophila
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additional information the cells are grown in the presence of the antibiotics vancomycin, cefotaxime, and chloramphenicol Chlamydomonas acidophila
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Synonyms

Synonyms Comment Organism
CaPCS1
-
 Chlamydomonas acidophila
CaPCS2
-
 Chlamydomonas acidophila
DaPCS1
-
 Dunaliella acidophila
PCS
-
 Dunaliella acidophila
PCS
-
 Chlamydomonas acidophila
phytochelatin synthase
-
 Dunaliella acidophila
phytochelatin synthase
-
 Chlamydomonas acidophila

Expression

Organism Comment Expression
Dunaliella acidophila the PCS gene in Dunaliella acidophila is not induced by cadmium, most likely due to gene duplication additional information
Chlamydomonas acidophila the PCS gene in Chlamydomonas acidophila shows very strong induction by cadmium up

General Information

General Information Comment Organism
evolution genes encoding phytochelatin synthases have been found in all vascular plants as well as some algae, fungi, diatoms and invertebrates. Horizontal gene transfer of phytochelatin synthases from bacteria to extremophilic green algae. A detailed phylogenetic analysis gives insight into the complicated evolutionary history of PCS genes and provides evidence for multiple horizontal gene transfer events from bacteria to eukaryotes within the gene family. A separate subgroup containing PCS-like genes within the PCS gene family is not supported since the PCS genes are monophyletic only when the PCS-like genes are included. Genotyping, overview Dunaliella acidophila
evolution genes encoding phytochelatin synthases have been found in all vascular plants as well as some algae, fungi, diatoms and invertebrates. Horizontal gene transfer of phytochelatin synthases from bacteria to extremophilic green algae. A detailed phylogenetic analysis gives insight into the complicated evolutionary history of PCS genes and provides evidence for multiple horizontal gene transfer events from bacteria to eukaryotes within the gene family. A separate subgroup containing PCS-like genes within the PCS gene family is not supported since the PCS genes are monophyletic only when the PCS-like genes are included. Genotyping, overview Chlamydomonas acidophila
physiological function phytochelatins (PCs) mediate high-affinity binding and contribute to detoxification of heavy metal ions and metalloids, such as cadmium or arsenic, by promoting the vacuo-lysosomal sequestration of heavy metals. These compounds are enzymatically synthesized from reduced glutathione (GSH) and related thiols in a gamma-glutamylcysteinyltranspeptidation reaction catalyzed by phytochelatin synthase (PCS) Chlamydomonas acidophila
physiological function phytochelatins (PCs) mediate high-affinity binding and contribute to detoxification of heavy metal ions and metalloids, such as cadmium or arsenic, by promoting the vacuolysosomal sequestration of heavy metals. These compounds are enzymatically synthesized from reduced glutathione (GSH) and related thiols in a gamma-glutamylcysteinyltranspeptidation reaction catalyzed by phytochelatin synthase (PCS) Dunaliella acidophila