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Literature summary for 2.3.2.13 extracted from
- Preparation and characteristics of gelatin sponges crosslinked by microbial transglutaminase (2017), PeerJ, 5, e3665 .
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | microbial transglutaminase (mTG) is used as a crosslinking agent in the preparation of gelatin sponges. The physical properties of the materials are evaluated by measuring their material porosity, water absorption, and elastic modulus. The stability of the sponges are assessed via hydrolysis and enzymolysis, overview. To evaluate the cell compatibility of them TG crosslinked gelatin sponges (mTG sponges), adipose-derived stromal stem cells are cultured and inoculated into the scaffold. Cell proliferation and viability are measured using alamarBlue assay and LIVE/DEAD fluorescence staining, respectively. Cell adhesion on the sponges is observed by scanning electron microscopy. mTG sponges have uniform pore size, high porosity and water absorption, and good mechanical properties. In subcutaneous implantation (in Sprague-Dawley rats), the material is partially degraded in the first month and completely absorbed in the third month. Cell experiments show evident cell proliferation and high viability. The cells grow vigorously and adhered tightly to the sponge. In conclusion, mTG sponge has good biocompatibility and can be used in tissue engineering and regenerative medicine | Streptomyces mobaraensis |
| medicine | microbial transglutaminase (mTG) is used as a crosslinking agent in the preparation of gelatin sponges. The physical properties of the materials are evaluated by measuring their material porosity, water absorption, and elastic modulus. The stability of the sponges are assessed via hydrolysis and enzymolysis, overview. To evaluate the cell compatibility of them TG crosslinked gelatin sponges (mTG sponges), adipose-derived stromal stem cells are cultured and inoculated into the scaffold. Cell proliferation and viability are measured using alamarBlue assay and LIVE/DEAD fluorescence staining, respectively. Cell adhesion on the sponges is observed by scanning electron microscopy. mTG sponges have uniform pore size, high porosity and water absorption, and good mechanical properties. In subcutaneous implantation (in Spraguex15Dawley rats), the material is partially degraded in the first month and completely absorbed in the third month. Cell experiments show evident cell proliferation and high viability. The cells grow vigorously and adhered tightly to the sponge. In conclusion, mTG sponge has good biocompatibility and can be used in tissue engineering and regenerative medicine | Streptomyces mobaraensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | mTG crosslinked gelatin hydrogel preparation | Streptomyces mobaraensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces mobaraensis | P81453 | - |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| microbial transglutaminase | - |
Streptomyces mobaraensis |
| MTG | - |
Streptomyces mobaraensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 37 | assay at | Streptomyces mobaraensis |
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