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Literature summary for 2.3.2.13 extracted from
- Purification and characterization of a high-salt-resistant microbial transglutaminase from Streptomyces mobaraensis (2016), J. Mol. Catal. B, 133, 6-11 .
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | transglutaminase MTG-TX can be used in food-related applications in salty environment, e.g. bacon or seafood | Streptomyces mobaraensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | strong inhibition | Streptomyces mobaraensis |  |
| Fe3+ | strong inhibition | Streptomyces mobaraensis | |
| additional information | isozyme MTG-TX is not inhibited by Li+, Na+, K+, Ca2+, Mg2+, Ba2+, Mn2+, or the inhibitor phenylmethanesulfonyl fluoride (PMSF) | Streptomyces mobaraensis | |
| N-ethylmaleimide | strong inhibition | Streptomyces mobaraensis | |
| Zn2+ | strong inhibition | Streptomyces mobaraensis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.37 | - |
hydroxylamine | pH 6.0, 37°C | Streptomyces mobaraensis | |
| 23.12 | - |
N-CBZ-Glu-Gly | pH 6.0, 37°C | Streptomyces mobaraensis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Streptomyces mobaraensis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is not affected by Cu2+ and Fe3+ | Streptomyces mobaraensis | |
| NaCl | purification of a high-salt resistent microbial transglutaminase, isozyme MTG-TX, 79.8% activity remaining | Streptomyces mobaraensis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein glutamine + alkylamine | Streptomyces mobaraensis | - |
protein N5-alkylglutamine + NH3 | - |
? | |
| protein glutamine + alkylamine | Streptomyces mobaraensis TX | - |
protein N5-alkylglutamine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces mobaraensis | P81453 | collected from soil | - |
| Streptomyces mobaraensis TX | P81453 | collected from soil | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of extracellular high-salt resistent microbial transglutaminase, isozyme MTG-TX, by ethanol precipitation, cation exchange chromatography, hydrophobic interaction chromatography, and gel filtration, method optimization, overview | Streptomyces mobaraensis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| protein glutamine + alkylamine = protein N5-alkylglutamine + NH3 | ping-pong mechanism | Streptomyces mobaraensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 39.2 | - |
purified extracellular isozyme MTG-TX, pH 6.0, 37°C | Streptomyces mobaraensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-CBZ-Glu-Gly + hydroxylamine | - |
Streptomyces mobaraensis | CBZ-Glu-(gamma-monohydroxamate)-Gly + NH3 | - |
? | |
| N-CBZ-Glu-Gly + hydroxylamine | - |
Streptomyces mobaraensis TX | CBZ-Glu-(gamma-monohydroxamate)-Gly + NH3 | - |
? | |
| protein glutamine + alkylamine | - |
Streptomyces mobaraensis | protein N5-alkylglutamine + NH3 | - |
? | |
| protein glutamine + alkylamine | - |
Streptomyces mobaraensis TX | protein N5-alkylglutamine + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37816, mass spectrometry | Streptomyces mobaraensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| microbial transglutaminase | - |
Streptomyces mobaraensis |
| MTG-TX | - |
Streptomyces mobaraensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 48 | - |
isozyme MTG-TX | Streptomyces mobaraensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | activity range, profile overview | Streptomyces mobaraensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the high-salt resistent isozyme MTG-TX exhibits a broad range of pH and temperature stability | Streptomyces mobaraensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.83 | - |
hydroxylamine | pH 6.0, 37°C | Streptomyces mobaraensis | |
| 1.83 | - |
N-CBZ-Glu-Gly | pH 6.0, 37°C | Streptomyces mobaraensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
isozyme MTG-TX | Streptomyces mobaraensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | activity range of isozyme MTG-TX | Streptomyces mobaraensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | the high-salt resistent isozyme MTG-TX exhibits a broad range of pH and temperature stability | Streptomyces mobaraensis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the enzyme shows a ping-pong mechanism | Streptomyces mobaraensis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.079 | - |
N-CBZ-Glu-Gly | pH 6.0, 37°C | Streptomyces mobaraensis | |
| 1.34 | - |
hydroxylamine | pH 6.0, 37°C | Streptomyces mobaraensis | |
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