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Literature summary for 2.3.2.13 extracted from
- In situ enzymatic activity of transglutaminase isoforms on brain tissue sections of rodents A new approach to monitor differences in post-translational protein modifications during neurodegeneration (2016), Brain Res., 1631, 22-33 .
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Mus musculus | 5737 | - |
| cytoplasm | - |
Rattus norvegicus | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Mus musculus |  |
| Ca2+ | required | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein glutamine + alkylamine | Mus musculus | - |
protein N5-alkylglutamine + NH3 | - |
? | |
| protein glutamine + alkylamine | Rattus norvegicus | - |
protein N5-alkylglutamine + NH3 | - |
? | |
| protein glutamine + alkylamine | Rattus norvegicus Sprague-Dawley | - |
protein N5-alkylglutamine + NH3 | - |
? | |
| protein glutamine + alkylamine | Mus musculus C57BL/6J | - |
protein N5-alkylglutamine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | P21981 | - |
- |
| Mus musculus | Q8BM11 | - |
- |
| Mus musculus C57BL/6J | P21981 | - |
- |
| Mus musculus C57BL/6J | Q8BM11 | - |
- |
| Rattus norvegicus | F1M4T7 | - |
- |
| Rattus norvegicus Sprague-Dawley | F1M4T7 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | in situ and in vitro enzymatic activity of transglutaminase isoforms on brain tissue sections and brain extract, monitoring of differences in post-translational protein modifications during neurodegeneration, overview. In situ activity of isoform TG6 varies between BACHD mice in comparison to their wild-type controls. TG6 protein expression is prominent in the brains of mice | Mus musculus | - |
| brain | in situ and in vitro enzymatic activity of transglutaminase isoforms on brain tissue sections and brain extract, monitoring of differences in post-translational protein modifications during neurodegeneration, overview. TG6 shows only a faint vascular signal as compared to a prominent cellular staining in rat tissue | Rattus norvegicus | - |
| cerebellum | - |
Mus musculus | - |
| cerebellum | - |
Rattus norvegicus | - |
| cerebral cortex | - |
Mus musculus | - |
| cerebral cortex | - |
Rattus norvegicus | - |
| additional information | TG6 expression seems to localize to neurons but not to astrocytes or microglia | Rattus norvegicus | - |
| additional information | TG6 expression seems to localize to neurons but not to astrocytes or microglia. TG6 shows only a faint vascular signal as compared to a prominent cellular staining in mouse tissue | Mus musculus | - |
| neuron | - |
Mus musculus | - |
| neuron | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| biotinyl-Aca-DDWDAMDEQIWF + alkylamine | a TG6 isozyme specific biotinylated peptide substrate | Mus musculus | ? | - |
? | |
| biotinyl-Aca-DDWDAMDEQIWF + alkylamine | a TG6 isozyme specific biotinylated peptide substrate | Rattus norvegicus | ? | - |
? | |
| biotinyl-Aca-DDWDAMDEQIWF + alkylamine | a TG6 isozyme specific biotinylated peptide substrate | Rattus norvegicus Sprague-Dawley | ? | - |
? | |
| biotinyl-Aca-DDWDAMDEQIWF + alkylamine | a TG6 isozyme specific biotinylated peptide substrate | Mus musculus C57BL/6J | ? | - |
? | |
| biotinyl-HQSYVDPWMLDH + alkylamine | a TG2 isozyme specific biotinylated peptide substrate | Mus musculus | ? | - |
? | |
| biotinyl-HQSYVDPWMLDH + alkylamine | a TG2 isozyme specific biotinylated peptide substrate | Mus musculus C57BL/6J | ? | - |
? | |
| additional information | evaluation of cross-reactivity of biotinylated peptides with recombinant isozymes TG2 and TG6. TG2 reactivity is significantly specific (i.e. higher than the cross-reactivity towards TG6-specific peptide) at either the lower and higher enzymatic concentrations used. Usage of a colorimetric assay for theTG-dependent epsilon-(gamma-glutamyl)lysine cross-linking reaction | Mus musculus | ? | - |
- |
|
| additional information | evaluation of cross-reactivity of biotinylated peptides with recombinant isozymes TG2 and TG6. TG6 reactivity is barely detectable at the lower concentration, and only appears to be measurable and specific at the higher dose. In both cases, reactivity measured against the other peptide is not significantly different than background signal measured in control wells. The used peptides are highly reactive and selective for their corresponding isoform. Usage of a colorimetric assay for the TG-dependent epsilon-(gamma-glutamyl)lysine cross-linking reaction | Mus musculus | ? | - |
- |
|
| additional information | evaluation of cross-reactivity of biotinylated peptides. TG-isoform-specific enzyme activity on rat brain tissue, overview. Usage of a colorimetric assay for the TG-dependent epsilon-(gamma-glutamyl)lysine cross-linking reaction | Rattus norvegicus | ? | - |
- |
|
| additional information | evaluation of cross-reactivity of biotinylated peptides. TG-isoform-specific enzyme activity on rat brain tissue, overview. Usage of a colorimetric assay for the TG-dependent epsilon-(gamma-glutamyl)lysine cross-linking reaction | Rattus norvegicus Sprague-Dawley | ? | - |
- |
|
| additional information | evaluation of cross-reactivity of biotinylated peptides with recombinant isozymes TG2 and TG6. TG2 reactivity is significantly specific (i.e. higher than the cross-reactivity towards TG6-specific peptide) at either the lower and higher enzymatic concentrations used. Usage of a colorimetric assay for theTG-dependent epsilon-(gamma-glutamyl)lysine cross-linking reaction | Mus musculus C57BL/6J | ? | - |
- |
|
| additional information | evaluation of cross-reactivity of biotinylated peptides with recombinant isozymes TG2 and TG6. TG6 reactivity is barely detectable at the lower concentration, and only appears to be measurable and specific at the higher dose. In both cases, reactivity measured against the other peptide is not significantly different than background signal measured in control wells. The used peptides are highly reactive and selective for their corresponding isoform. Usage of a colorimetric assay for the TG-dependent epsilon-(gamma-glutamyl)lysine cross-linking reaction | Mus musculus C57BL/6J | ? | - |
- |
|
| protein glutamine + alkylamine | - |
Mus musculus | protein N5-alkylglutamine + NH3 | - |
? | |
| protein glutamine + alkylamine | - |
Rattus norvegicus | protein N5-alkylglutamine + NH3 | - |
? | |
| protein glutamine + alkylamine | - |
Rattus norvegicus Sprague-Dawley | protein N5-alkylglutamine + NH3 | - |
? | |
| protein glutamine + alkylamine | - |
Mus musculus C57BL/6J | protein N5-alkylglutamine + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mammalian transglutaminase | - |
Mus musculus |
| mammalian transglutaminase | - |
Rattus norvegicus |
| TG2 | - |
Mus musculus |
| TG6 | - |
Mus musculus |
| TG6 | - |
Rattus norvegicus |
| transglutaminase | - |
Mus musculus |
| transglutaminase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Mus musculus |
| 8.5 | - |
assay at | Rattus norvegicus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | mammalian transglutaminases (TGs) catalyze irreversible posttranslational modifications of proteins, the most prominent of which is the calcium-dependent formation of covalent acyl transfers between the gamma-carboxamide group of glutamine and the epsilon-amino-group of lysine (GGEL-linkage) | Mus musculus |
| physiological function | mammalian transglutaminases (TGs) catalyze irreversible posttranslational modifications of proteins, the most prominent of which is the calcium-dependent formation of covalent acyl transfers between the gamma-carboxamide group of glutamine and the epsilon-amino-group of lysine (GGEL-linkage) | Rattus norvegicus |
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Release 2023.1 (January 2023)
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