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Literature summary for 2.3.2.13 extracted from
- Molecular insights into the mechanism of substrate recognition of Streptomyces transglutaminases (2020), Biosci. Biotechnol. Biochem., 84, 575-582 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and chimeric mutant enzymes in Streptomyces lividans strain 1326, Streptomyces TGase is secreted as a zymogen with an additional prosequence at the N-terminus | Streptomyces cinnamoneus |
| recombinant expression of wild-type and chimeric mutant enzymes in Streptomyces lividans strain 1326, Streptomyces TGase is secreted as a zymogen with an additional prosequence at the N-terminus | Streptomyces mobaraensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a chimeric mutant constructed from the TGases of Streptomyces mobaranensis (SMTG) and Streptomyces cinnamoneus (SCTG), the mutant enzyme consists of the N-terminal half of SCTG and the C-terminal half of SMTG | Streptomyces mobaraensis |
| additional information | construction of a chimeric mutant constructed from the TGases of Streptomyces mobaranensis (SMTG) and Streptomyces cinnamoneus (SCTG), the mutant enzyme consists of the N-terminal half of SCTG and the C-terminal half of SMTG | Streptomyces cinnamoneus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.9 | - |
L-lysine | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces cinnamoneus |  |
| 4.5 | - |
L-lysine | pH 8.0, 37°C, recombinant chimeric mutant | Streptomyces mobaraensis | |
| 4.5 | - |
L-lysine | pH 8.0, 37°C, recombinant chimeric mutant | Streptomyces cinnamoneus | |
| 6.4 | - |
L-lysine | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces cinnamoneus | |
| 6.5 | - |
L-lysine | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces mobaraensis | |
| 7.8 | - |
hydroxylamine | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces mobaraensis | |
| 9.9 | - |
L-lysine | pH 8.0, 37°C, recombinant chimeric mutant | Streptomyces mobaraensis | |
| 9.9 | - |
L-lysine | pH 8.0, 37°C, recombinant chimeric mutant | Streptomyces cinnamoneus | |
| 15.8 | - |
L-lysine | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces mobaraensis | |
| 19.6 | - |
hydroxylamine | pH 8.0, 37°C, recombinant chimeric mutant | Streptomyces mobaraensis | |
| 19.6 | - |
hydroxylamine | pH 8.0, 37°C, recombinant chimeric mutant | Streptomyces cinnamoneus | |
| 61.9 | - |
hydroxylamine | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces cinnamoneus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein glutamine + alkylamine | Streptomyces mobaraensis | - |
protein N5-alkylglutamine + NH3 | - |
? | |
| protein glutamine + alkylamine | Streptomyces cinnamoneus | - |
protein N5-alkylglutamine + NH3 | - |
? | |
| protein glutamine + alkylamine | Streptomyces cinnamoneus NBRC 13864 | - |
protein N5-alkylglutamine + NH3 | - |
? | |
| protein glutamine + alkylamine | Streptomyces mobaraensis NBRC 13819 | - |
protein N5-alkylglutamine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces cinnamoneus | Q8GR90 | - |
- |
| Streptomyces cinnamoneus NBRC 13864 | Q8GR90 | - |
- |
| Streptomyces mobaraensis | P81453 | - |
- |
| Streptomyces mobaraensis NBRC 13819 | P81453 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | Streptomyces TGase is secreted as a zymogen with an additional prosequence at the N-terminus, the cleavage of the prosequence activates the enzyme | Streptomyces mobaraensis |
| proteolytic modification | Streptomyces TGase is secreted as a zymogen with an additional prosequence at the N-terminus, the cleavage of the prosequence activates the enzyme | Streptomyces cinnamoneus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble enzyme from cell-free supernatant of Streptomyces lividans strain 1326 by ammonium sulfate fractionation, dialysis, and ultrafiltration | Streptomyces mobaraensis |
| recombinant soluble enzyme from cell-free supernatant of Streptomyces lividans strain 1326 by ammonium sulfate fractionation, dialysis, and ultrafiltration | Streptomyces cinnamoneus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Cbz-Gln-Gly + D-lysine | - |
Streptomyces mobaraensis | Cbz-N5-aminocaproyl-glutaminyl-Gly + NH3 | - |
? | |
| Cbz-Gln-Gly + D-lysine | - |
Streptomyces cinnamoneus | Cbz-N5-aminocaproyl-glutaminyl-Gly + NH3 | - |
? | |
| Cbz-Gln-Gly + D-lysine | - |
Streptomyces cinnamoneus NBRC 13864 | Cbz-N5-aminocaproyl-glutaminyl-Gly + NH3 | - |
? | |
| Cbz-Gln-Gly + D-lysine | - |
Streptomyces mobaraensis NBRC 13819 | Cbz-N5-aminocaproyl-glutaminyl-Gly + NH3 | - |
? | |
| Cbz-Gln-Gly + hydroxylamine | - |
Streptomyces mobaraensis | Cbz-N5-hydroxyglutaminyl-Gly + NH3 | - |
? | |
| Cbz-Gln-Gly + hydroxylamine | - |
Streptomyces cinnamoneus | Cbz-N5-hydroxyglutaminyl-Gly + NH3 | - |
? | |
| Cbz-Gln-Gly + hydroxylamine | - |
Streptomyces cinnamoneus NBRC 13864 | Cbz-N5-hydroxyglutaminyl-Gly + NH3 | - |
? | |
| Cbz-Gln-Gly + hydroxylamine | - |
Streptomyces mobaraensis NBRC 13819 | Cbz-N5-hydroxyglutaminyl-Gly + NH3 | - |
? | |
| Cbz-Gln-Gly + L-lysine | - |
Streptomyces mobaraensis | Cbz-N5-aminocaproyl-glutaminyl-Gly + NH3 | - |
? | |
| Cbz-Gln-Gly + L-lysine | - |
Streptomyces cinnamoneus | Cbz-N5-aminocaproyl-glutaminyl-Gly + NH3 | - |
? | |
| Cbz-Gln-Gly + L-lysine | - |
Streptomyces cinnamoneus NBRC 13864 | Cbz-N5-aminocaproyl-glutaminyl-Gly + NH3 | - |
? | |
| Cbz-Gln-Gly + L-lysine | - |
Streptomyces mobaraensis NBRC 13819 | Cbz-N5-aminocaproyl-glutaminyl-Gly + NH3 | - |
? | |
| additional information | TGase activity on the productivity of crosslinking peptide with tryptic casein, substrate specificity of wild-type and mutant enzymes, overview | Streptomyces mobaraensis | ? | - |
- |
|
| additional information | TGase activity on the productivity of crosslinking peptide with tryptic casein, substrate specificity of wild-type and mutant enzymes, overview | Streptomyces cinnamoneus | ? | - |
- |
|
| additional information | TGase activity on the productivity of crosslinking peptide with tryptic casein, substrate specificity of wild-type and mutant enzymes, overview | Streptomyces cinnamoneus NBRC 13864 | ? | - |
- |
|
| additional information | TGase activity on the productivity of crosslinking peptide with tryptic casein, substrate specificity of wild-type and mutant enzymes, overview | Streptomyces mobaraensis NBRC 13819 | ? | - |
- |
|
| protein glutamine + alkylamine | - |
Streptomyces mobaraensis | protein N5-alkylglutamine + NH3 | - |
? | |
| protein glutamine + alkylamine | - |
Streptomyces cinnamoneus | protein N5-alkylglutamine + NH3 | - |
? | |
| protein glutamine + alkylamine | - |
Streptomyces cinnamoneus NBRC 13864 | protein N5-alkylglutamine + NH3 | - |
? | |
| protein glutamine + alkylamine | - |
Streptomyces mobaraensis NBRC 13819 | protein N5-alkylglutamine + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SCTG | - |
Streptomyces cinnamoneus |
| SMTG | - |
Streptomyces mobaraensis |
| transglutaminase | - |
Streptomyces mobaraensis |
| transglutaminase | - |
Streptomyces cinnamoneus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
wild-type enzyme | Streptomyces cinnamoneus |
| 45.4 | - |
chimeric mutant enzyme | Streptomyces mobaraensis |
| 45.4 | - |
chimeric mutant enzyme | Streptomyces cinnamoneus |
| 50.9 | - |
wild-type enzyme | Streptomyces mobaraensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45.8 | - |
Tm of the wild-type enzyme | Streptomyces cinnamoneus |
| 46 | - |
Tm of the chimeric mutant enzyme | Streptomyces mobaraensis |
| 46 | - |
Tm of the chimeric mutant enzyme | Streptomyces cinnamoneus |
| 51.7 | - |
Tm of the wild-type enzyme | Streptomyces mobaraensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
wild-type enzyme | Streptomyces cinnamoneus |
| 9 | - |
chimeric mutant enzyme | Streptomyces cinnamoneus |
| 9 | - |
wild-type and chimeric mutant enzymes | Streptomyces mobaraensis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | comparison of the different TGases from Streptomyces mobaranensis and Streptomyces cinnamoneus, and of a chimeric mutant constructed from both, overview | Streptomyces mobaraensis |
| additional information | comparison of the different TGases from Streptomyces mobaranensis and Streptomyces cinnamoneus, and of a chimeric mutant constructed from both, overview | Streptomyces cinnamoneus |
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