Literature summary for 2.3.2.13 extracted from

Mu, D.; Lu, J.; Shu, C.; Li, H.; Li, X.; Cai, J.; Luo, S.; Yang, P.; Jiang, S.; Zheng, Z.
Improvement of the activity and thermostability of microbial transglutaminase by multiple-site mutagenesis (2018), Biosci. Biotechnol. Biochem., 82, 106-109 .

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Application

Application	Comment	Organism
food industry	microbial transglutaminase (MTG) is an enzyme widely used in the food industry	Streptomyces mobaraensis

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of wild-type and mutant enzymes in Escherichia coli	Streptomyces mobaraensis

Protein Variants

Protein Variants	Comment	Organism
additional information	mutiple-site mutagenesis of Streptomyces mobaraensis transglutaminase is performed in Escherichia coli. According to enzymatic assay and thermostability study, among three penta-site MTG mutants (DM01-03), DM01 exhibits the highest enzymatic activity of 55.7 U/mg and longest half-life at 50°C (418.2 min) and 60°C (24.8 min)	Streptomyces mobaraensis

Organism

Organism	UniProt	Comment	Textmining
Streptomyces mobaraensis	P81453	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
55.7	-	purified recombinant enzyme penta-site mutant DM01, pH and temperature not specified in the publication	Streptomyces mobaraensis

Synonyms

Synonyms	Comment	Organism
microbial transglutaminase	-	Streptomyces mobaraensis
MTG	-	Streptomyces mobaraensis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	purified recombinant enzyme penta-site mutant DM01, half-life is 418.2 min	Streptomyces mobaraensis
60	-	purified recombinant enzyme penta-site mutant DM01, half-life is 24.8 min	Streptomyces mobaraensis

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Release 2023.1 (January 2023)