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Literature summary for 2.3.2.13 extracted from
- Identification of DNAJA1 as a novel interacting partner and a substrate of human transglutaminase 2 (2016), Biochem. J., 473, 3889-3901 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | DNAJA1 facilitates and stabilizes in vitro cross-linking activity of TG2. The molecular co-chaperone, DNAJA1, is an interacting partner of human isozyme TG2. DNAJA1 interacts with the open form of TG2 and regulates its transamidation activity under both in vitro and in situ conditions. DNAJA1 does not affect the enzyme activity. DNAJA1 and TG2 are reported to regulate common pathological conditions such as neurodegenerative disorders and cancer, molecular mechanisms, interaction analysis, overview | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of GST-tagged full-length TG2 V224 and truncated G224 variants in Escherichia coli strain Rosetta 2 (DE3) | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of stable cell lines of HEK-293T AD cells overexpressing human TG2. Generation of domain variants of TG2, i.e. GST-TG2DELTAbeta-barrel2, full-length TG2, GST-CAT, GST-TG2DELTAbeta-sandwich, GST-TG2DELTAbeta-barrel1, GST-TG2DELTACAT | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the kinetic parameters of TG2 for DMC substrate in the presence and absence of DNAJA1 are estimated by Michaelis-Menten and Lineweaver-Burk plots | Homo sapiens | |
| 0.0176 | - |
N,N'-dimethyl casein glutamine | pH 7.5, 37°C, recombinant TG2 without DNAJA1 | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein glutamine + alkylamine | Homo sapiens | - |
protein N5-alkylglutamine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P21980 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged full-length TG2 V224 and G224 variants from Escherichia coli strain Rosetta 2 (DE3) by glutathione affinity chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N,N'-dimethyl casein glutamine + monodansylcadaverine | the enzymatic activity of TG2 is determined by measuring the fluorescence of monodansylcadaverine (MDC) crosslinking to N,N'-dimethyl casein upon exposure | Homo sapiens | ? | - |
? | |
| protein glutamine + alkylamine | - |
Homo sapiens | protein N5-alkylglutamine + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TG2 | - |
Homo sapiens |
| transglutaminase 2 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
N,N'-dimethyl casein glutamine | pH 7.5, 37°C, recombinant TG2 without DNAJA1 | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | transglutaminase 2 (TG2) is a ubiquitously expressed multifunctional member of the transglutaminase enzyme family | Homo sapiens |
| metabolism | transglutaminase 2 (TG2) is a ubiquitously expressed multifunctional member of the transglutaminase enzyme family. It is implicated to have roles in many physiological and pathological processes such as differentiation, apoptosis, signal transduction, adhesion and migration, wound healing and inflammation. TG2 has various intra- and extra-cellular interacting partners, which contribute to these processes. The molecular co-chaperone, DNAJA1, is an interacting partner of human isozyme TG2. DNAJA1 and TG2 are reported to regulate common pathological conditions such as neurodegenerative disorders and cancer | Homo sapiens |
| additional information | the catalytic core domain of TG2 is essential for the TG2-DNAJA1 interaction, mass spectrometry analysis. In contrast, the cross-linking activity of TG2 is not essential for the interaction since DNAJA1 also interacts with the catalytically inactive form of TG2. DNAJA1 interacts with the open form of TG2 and regulates its transamidation activity under both in vitro and in situ conditions | Homo sapiens |
| physiological function | DNAJA1 interacts with the open form of TG2 and regulates its transamidation activity under both in vitro and in situ conditions. DNAJA1 and TG2 are reported to regulate common pathological conditions such as neurodegenerative disorders and cancer, molecular mechanisms, overview | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 85.2 | - |
N,N'-dimethyl casein glutamine | pH 7.5, 37°C, recombinant TG2 without DNAJA1 | Homo sapiens |
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