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Literature summary for 2.3.2.13 extracted from

  • Liu, Y.; Huang, L.; Shan, M.; Sang, J.; Li, Y.; Jia, L.; Wang, N.; Wang, S.; Shao, S.; Liu, F.; Lu, F.
    Enhancing the activity and thermostability of Streptomyces mobaraensis transglutaminase by directed evolution and molecular dynamics simulation (2019), Biochem. Eng. J., 151, 107333 .
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Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type and mutant enzymes in Corynebacterium glutamicum strain ATCC 13032, subcloning in Escherichia coli strain DH5alpha Streptomyces mobaraensis

Protein Variants

Protein Variants Comment Organism
E164L site-directed mutagenesis, the E164L mutant exhibits a 1.95fold increased specific activity and 1.66fold increased half-life at 50°C compared to wild-type. The molecular dynamics (MD) simulation results indicate that the mutation Glu164Leu results in weaker interactions of Asp159-Glu164 and Gly228-Leu231, leading to the enhanced instability of Ile240-Asn253 linked to Gly228-Leu231 by eight residues. It further causes reduced interactions between loop region 1 (Ile240-Asn253) and loop region 2 (His277-Met288), facilitating the access of substrate molecule to the active site. Structure-activity relationship for MTG adapted to high temperature conditions. Enhancing activity and thermostability of Streptomyces mobaraensis transglutaminase by directed evolution, Molecular mechanism of improved activity of E164L analyzed by molecular dynamics simulations Streptomyces mobaraensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein glutamine + alkylamine Streptomyces mobaraensis
-
 protein N5-alkylglutamine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces mobaraensis P81453
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-CBZ-Glu-Gly + hydroxylamine
-
 Streptomyces mobaraensis CBZ-Glu-(gamma-monohydroxamate)-Gly + NH3
-
 ?
protein glutamine + alkylamine
-
 Streptomyces mobaraensis protein N5-alkylglutamine + NH3
-
 ?

Synonyms

Synonyms Comment Organism
MTG
-
 Streptomyces mobaraensis
transglutaminase
-
 Streptomyces mobaraensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 recombinant enzyme Streptomyces mobaraensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 activity range, wild-type and mutant enzyme, profiles overview Streptomyces mobaraensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 after treatment at 50°C and pH 7.0 for 60, 80, 100, and 120 min, respectively, recombinant mutant E164L maintains 60%, 41%, 29%, and 16% of its original activity Streptomyces mobaraensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 recombinant enzyme Streptomyces mobaraensis

pH Range

pH Minimum pH Maximum Comment Organism
4 9 activity range, wild-type and mutant enzyme, profiles overview Streptomyces mobaraensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 after treatment at 50°C and pH 7.0 for 60, 80, 100, and 120 min, respectively, recombinant mutant E164L maintains 60%, 41%, 29%, and 16% of its original activity Streptomyces mobaraensis

General Information

General Information Comment Organism
additional information molecular dynamics simulations Streptomyces mobaraensis