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Literature summary for 2.3.2.13 extracted from

  • Pinkas, D.M.; Strop, P.; Brunger, A.T.; Khosla, C.
    Transglutaminase 2 undergoes a large conformational change upon activation (2007), PLoS Biol., 5, e327.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization of the enzyme inhibited with Ac-P(6-diazo-5-oxo-L-norleucine)LPF-NH2, hanging-drop method at 25°C, 2 A resolution Homo sapiens

Protein Variants

Protein Variants Comment Organism
T360A mutants show an increase in preference for deamidation with respect to transamidation compared to the wild-type enzyme Homo sapiens
T360W mutants show an increase in preference for deamidation with respect to transamidation compared to the wild-type enzyme Homo sapiens
W241A no detectable activity Homo sapiens
W332A no detectable activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Ac-P(6-diazo-5-oxo-L-norleucine)LPF-NH2 high-affinity irreversible inhibitor of TG2. The inhibitor stabilizes TG2 in an extended conformation that is dramatically different from earlier transglutaminase structures. The active site is exposed, revealing that catalysis takes place in a tunnel, bridged by two tryptophan residues that separate acyl-donor from acyl-acceptor and stabilize the tetrahedral reaction intermediates Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic parameters of wild-type TG2, T360W, and T360A mutants using Ac-PQLPF-NH2 as the acyl-donor and putrescine as the acyl-acceptor Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Homo sapiens P21980
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-PQLPF-NH2 + putrescine
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
TG2
-
Homo sapiens
transglutaminase 2
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information kinetic parameters of wild-type TG2, T360W, and T360A mutants using Ac-PQLPF-NH2 as the acyl-donor and putrescine as the acyl-acceptor Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00006
-
Ac-P(6-diazo-5-oxo-L-norleucine)LPF-NH2
-
Homo sapiens