Crystallization (Comment) | Organism |
---|---|
crystallization of the enzyme inhibited with Ac-P(6-diazo-5-oxo-L-norleucine)LPF-NH2, hanging-drop method at 25°C, 2 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
T360A | mutants show an increase in preference for deamidation with respect to transamidation compared to the wild-type enzyme | Homo sapiens |
T360W | mutants show an increase in preference for deamidation with respect to transamidation compared to the wild-type enzyme | Homo sapiens |
W241A | no detectable activity | Homo sapiens |
W332A | no detectable activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ac-P(6-diazo-5-oxo-L-norleucine)LPF-NH2 | high-affinity irreversible inhibitor of TG2. The inhibitor stabilizes TG2 in an extended conformation that is dramatically different from earlier transglutaminase structures. The active site is exposed, revealing that catalysis takes place in a tunnel, bridged by two tryptophan residues that separate acyl-donor from acyl-acceptor and stabilize the tetrahedral reaction intermediates | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters of wild-type TG2, T360W, and T360A mutants using Ac-PQLPF-NH2 as the acyl-donor and putrescine as the acyl-acceptor | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P21980 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-PQLPF-NH2 + putrescine | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TG2 | - |
Homo sapiens |
transglutaminase 2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters of wild-type TG2, T360W, and T360A mutants using Ac-PQLPF-NH2 as the acyl-donor and putrescine as the acyl-acceptor | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00006 | - |
Ac-P(6-diazo-5-oxo-L-norleucine)LPF-NH2 | - |
Homo sapiens |