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Literature summary for 2.3.2.13 extracted from

  • Cleary, D.B.; Maurer, M.C.
    Characterizing the specificity of activated Factor XIII for glutamine-containing substrate peptides (2006), Biochim. Biophys. Acta, 1764, 1207-1217.
    View publication on PubMed
Search for more literature for 2.3.2.13

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0409
-
 beta-casein
-
 Homo sapiens
0.208
-
 NQENVSPLTLLRLGN 37°C, pH 7.4 Homo sapiens
0.459
-
 NQEQVSPLTLLKLGN 37°C, pH 7.4 Homo sapiens
0.782
-
 LGPQSLVIG 37°C, pH 7.4 Homo sapiens
1.28
-
 NQENVSPLTLLKLGN 37°C, pH 7.4 Homo sapiens
1.43
-
 LGPQSKVIG 37°C, pH 7.4 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 recombinant factor XIIIa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-casein + glycine ethylester
-
 Homo sapiens ?
-
 ?
LGPQSKVIG + glycine-ethylester i.e. K9, an optimized sequence based on beta-casein Homo sapiens ?
-
 ?
LGPQSLVIG + glycine ethylester i.e. K9(K7L), a modified optimized sequence based on beta-casein Homo sapiens ?
-
 ?
additional information study of enzyme specificity with Q-containing substrates based on beta-casein, K9-peptide and alpha2-antiplasmin. Factor XIIIa preferentially selects the Q2 residue for carrying out crosslinking processes.The E3 and Q4 provide supporting role in binding. When reaction occurs at Q2, then Q4 is sterically blocked. Deamidation of Q2 to E2 allows observation of reactivity at Q4. K12 position provides an additional favorable site of interaction with factor XIIIa surface Homo sapiens ?
-
 ?
NNEQVSPLTLLKLGN + glycine ethylester i.e. alpha2-antiplasmin peptide with modification Q2N Homo sapiens ?
-
 ?
NQENVSPLTLLKLGN + glycine ethylester i.e. alpha2-antiplasmin peptide with modification Q4N Homo sapiens ?
-
 ?
NQENVSPLTLLLLGN + glycine ethylester i.e. alpha2-antiplasmin peptide with modification Q4N, K12L Homo sapiens ?
-
 ?
NQENVSPLTLLRLGN + glycine ethylester i.e. alpha2-antiplasmin peptide with modification Q4N, K12R Homo sapiens ?
-
 ?
NQEQVSPLTLLKLGN + glycine ethylester i.e. alpha2-antiplasmin peptide Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8
-
 NQENVSPLTLLRLGN 37°C, pH 7.4 Homo sapiens
16.1
-
 beta-casein
-
 Homo sapiens
44
-
 LGPQSLVIG 37°C, pH 7.4 Homo sapiens
77.3
-
 NQENVSPLTLLRLGN 37°C, pH 7.4 Homo sapiens
78
-
 LGPQSKVIG 37°C, pH 7.4 Homo sapiens
147
-
 NQENVSPLTLLKLGN 37°C, pH 7.4 Homo sapiens
175
-
 NQEQVSPLTLLKLGN 37°C, pH 7.4 Homo sapiens