Cloned (Comment) | Organism |
---|---|
expression of transglutaminase 3 in Sf9 cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion, 15 mg/ml transglutaminase 3, 0.1 mM beta-octylglucoside in 0.1 ml of enzyme in 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 125 mM NaCl and 3 mM CaCl2, well solution containing 4-12% polyethylene glycol 6K, 100 mM bicine, pH 9.0, and 1% dioxane at 21°C, X-ray structure of zymogen and activated transglutaminase 3 at 2.2 and 2.1 A resolution | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | transglutaminase 3 binds 3 Ca2+ ions, Er3+, Sm3+, Tb3+ and Lu3+ can substitute for Ca2+ to retain activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q08188 | - |
- |