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Literature summary for 2.3.2.13 extracted from

  • Murthy, S.N.P.; Velasco, P.T.; Lorand, L.
    Properties of purified lens transglutaminase and regulation of its transamidase/crosslinking activity by GTP (1998), Exp. Eye Res., 67, 273-281.
    View publication on PubMed
Search for more literature for 2.3.2.13

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
 Cavia porcellus
dithiothreitol required for activity of lens transglutaminase Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
GTP
-
 Cavia porcellus
GTP
-
 Homo sapiens
GTP 0.05 mM, 50% inhibition of lens transglutaminase, 0.5 mM, complete inhibition in the presence of 0.5 mM Ca2+, increasing the Ca2+ concentration to 3 mM reverses inhibition Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ requirement Cavia porcellus
Ca2+ requirement Homo sapiens
Ca2+ requirement Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
 x * 78000, lens transglutaminase, SDS-PAGE Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-
Homo sapiens
-
-
-
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
lens transglutaminase, DE-52, affinity chromatography on 42000 Da thermolytic fragment of human plasma fibronectin Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
lens
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
monodansylcadaverine + N,N-dimethylcasein
-
 Oryctolagus cuniculus ?
-
 ?
protein-bound gamma-glutamine + alkylamine
-
 Cavia porcellus protein N5-alkylglutamine + NH3
-
 ?
protein-bound gamma-glutamine + alkylamine
-
 Homo sapiens protein N5-alkylglutamine + NH3
-
 ?
protein-bound gamma-glutamine + alkylamine lens transglutaminase, crosslinking of beta-crystallin Oryctolagus cuniculus protein N5-alkylglutamine + NH3
-
 ?

Subunits

Subunits Comment Organism
?
-
 Homo sapiens
? x * 78000, lens transglutaminase, SDS-PAGE Oryctolagus cuniculus