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Literature summary for 2.3.2.13 extracted from

  • Slife, C.W.; Morris, G.S.; Snedeker, S.W.
    Solubilization and properties of the liver plasma membrane transglutaminase (1987), Arch. Biochem. Biophys., 257, 39-47.
    View publication on PubMed
Search for more literature for 2.3.2.13

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
 Rattus norvegicus

General Stability

General Stability Organism
Ca2+ stabilizes in combination with dithiothreitol Rattus norvegicus
dialysis against Ca2+-free buffers inactivates Rattus norvegicus
DTT stabilizes Rattus norvegicus
EDTA stabilizes in combination with dithiothreitol Rattus norvegicus
glycerol, 50% v/v, solubilizes and stabilizes Rattus norvegicus
ion-exchange chromatography on DEAE-cellulose, with 0.4 M NaCl containing buffer with or without Ca2+, stable to Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic studies of membrane-associated and soluble enzyme Rattus norvegicus
additional information
-
 additional information Km of N,N'-dimethylcasein: 0.44-0.52 mg/ml Rattus norvegicus
additional information
-
 additional information membrane-associated liver transglutaminase, Km for N,N'-dimethylcasein: 0.44 mg/ml, soluble transglutaminase, 0.52 mg/ml Rattus norvegicus
0.04
-
 putrescine soluble liver transglutaminase Rattus norvegicus
0.04 0.05 putrescine
-
 Rattus norvegicus
0.044
-
 putrescine membrane-associated liver transglutaminase Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane liver transglutaminase, plasma-membrane associated, lateral domain Rattus norvegicus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ requirement Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 male Sasco/King (SD)BR strain
-
Rattus norvegicus Sasco
-
 male Sasco/King (SD)BR strain
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus
selectively solubilized with glycerol Rattus norvegicus
Triton X-100, deoxycholate or n-octylglucoside only 20-30% effective Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Storage Stability

Storage Stability Organism
4°C, membrane preparation, rapid inactivation, DTT protects in combination with Ca2+ or EDTA Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
putrescine + N,N'-dimethylcasein
-
 Rattus norvegicus ?
-
 ?
putrescine + N,N'-dimethylcasein
-
 Rattus norvegicus Sasco ?
-
 ?