Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.1 M bis-Tris-HCl pH 6.5, 25% (w/v) PEG 3350 | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptidyl-tRNA1 + aminoacyl-tRNA2 | Mycobacterium tuberculosis | - |
tRNA1 + peptidyl(aminoacyl-tRNA2) | - |
? | |
peptidyl-tRNA1 + aminoacyl-tRNA2 | Mycobacterium tuberculosis CDC 1551 | - |
tRNA1 + peptidyl(aminoacyl-tRNA2) | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | O53223 | - |
- |
Mycobacterium tuberculosis CDC 1551 | O53223 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and Superdex 200 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptidyl-tRNA1 + aminoacyl-tRNA2 | - |
Mycobacterium tuberculosis | tRNA1 + peptidyl(aminoacyl-tRNA2) | - |
? | |
peptidyl-tRNA1 + aminoacyl-tRNA2 | - |
Mycobacterium tuberculosis CDC 1551 | tRNA1 + peptidyl(aminoacyl-tRNA2) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L,D-transpeptidase | - |
Mycobacterium tuberculosis |
LdtMt2 | - |
Mycobacterium tuberculosis |