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Literature summary for 2.3.2.12 extracted from

  • Thompson, H.A.; Moldave, K.
    Characterization of the peptidyltransferase reaction catalyzed by rat liver 60S ribosomal subunits (1974), Biochemistry, 13, 1348-1353.
    View publication on PubMed
Search for more literature for 2.3.2.12

Activating Compound

Activating Compound Comment Organism Structure
methanol about 30-40% concentration Rattus norvegicus
poly-U with acetylphenylalanyl-tRNA as donor only Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
40S subunits of ribosomes inhibition proportional to the 40S-subunit-concentration Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000058
-
 acetylphenylalanyl-tRNA
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
ribosome 60S subunit Rattus norvegicus 5840
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylphenylalanyl-tRNA + puromycin
-
 Rattus norvegicus acetylphenylalanyl-puromycin + tRNA
-
 ?
AcPhe-tRNA + puromycin
-
 Rattus norvegicus tRNA + AcPhe-puromycin
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 enzyme assay Rattus norvegicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 30
-
 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.25
-
 Rattus norvegicus