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Literature summary for 2.3.2.12 extracted from
- Release of lysine peptides by puromycin from polylysyl-transfer ribonucleic acid in the presence of ribosomes (1966), Biochim. Biophys. Acta, 114, 425-427.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NH4+ | - |
Escherichia coli |  |
| poly-A | - |
Escherichia coli | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chloramphenicol | - |
Escherichia coli | |
| erythromycin | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| ribosome | - |
Escherichia coli | 5840 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| peptidyl-tRNA1 + alpha-aminoacyl-tRNA2 | Escherichia coli | - |
tRNA1 + peptidyl-amino-tRNA2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
B cells | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| peptidyl-tRNA1 + alpha-aminoacyl-tRNA2 | - |
Escherichia coli | tRNA1 + peptidyl-amino-tRNA2 | - |
? | |
| polylysyl-tRNA + puromycin | - |
Escherichia coli | tRNA + polylysyl-puromycin | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
enzyme assay | Escherichia coli |
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