Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants as His-tagged proteins in Escherichia coli, in vitro transcription of tRNAs | Weissella viridescens |
Protein Variants | Comment | Organism |
---|---|---|
D109N | site-directed mutagenesis, loss of 99% activity compared to the wild-type | Weissella viridescens |
E205Q | site-directed mutagenesis, unaltered activity compared to the wild-type | Weissella viridescens |
E215Q | site-directed mutagenesis, loss of 15% activity compared to the wild-type | Weissella viridescens |
E316Q | site-directed mutagenesis, loss of 60% activity compared to the wild-type | Weissella viridescens |
E320Q | site-directed mutagenesis, loss of 96% activity compared to the wild-type | Weissella viridescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ser-tRNA | versus UDP-acetylmuramoylpentapeptide | Weissella viridescens | |
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine | product inhibition | Weissella viridescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
L-alanyl-tRNA(Ala) | Ala-tRNAAla from E. coli, recombinant wild-type | Weissella viridescens | |
0.042 | - |
UDP-acetylmuramoylpentapeptide | recombinant wild-type | Weissella viridescens | |
0.054 | - |
L-alanyl-tRNA(Ala) | Ala-tRNAAla from E. coli, recombinant mutant E320Q | Weissella viridescens | |
0.061 | - |
L-alanyl-tRNA(Ala) | Ala-tRNAAla from E. coli, recombinant mutant D109N | Weissella viridescens | |
0.067 | - |
UDP-acetylmuramoylpentapeptide | recombinant mutant E320Q | Weissella viridescens | |
0.165 | - |
UDP-acetylmuramoylpentapeptide | recombinant mutant D109N | Weissella viridescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Weissella viridescens | - |
femX gene | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutants from E. coli | Weissella viridescens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine = tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine | transfer of L-Ala to epsilon-amino group of L-lysine of UDP-UDP-acetylmuramoylpentapeptide in an ordered sequential mechanism | Weissella viridescens | |
L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine = tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine | catalysis via a ternary complex | Weissella viridescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanyl-tRNA(Ala) + UDP-N-acetylmuramoyl-pentapeptide containing L-lysine | - |
Weissella viridescens | ? | - |
r | |
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine | - |
Weissella viridescens | tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine | - |
? | |
L-alanyl-tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine | - |
Weissella viridescens | tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine | - |
? | |
L-seryl-tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine | - |
Weissella viridescens | tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-seryl)-L-lysyl-D-alanyl-D-alanine | - |
? | |
additional information | also utilizes UDP-N-acetylmuramoylpentapeptide containing L-ornithine as substrate | Weissella viridescens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Weissella viridescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.183 | - |
L-alanyl-tRNAAla | recombinant mutant D109N | Weissella viridescens | |
0.917 | - |
L-alanyl-tRNAAla | recombinant mutant E320Q | Weissella viridescens | |
11 | - |
L-alanyl-tRNAAla | recombinant wild-type | Weissella viridescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
broad maximum | Weissella viridescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pK values of 5.5 and 9.3 | Weissella viridescens |
5.5 | 9.1 | - |
Weissella viridescens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Weissella viridescens | |
0.048 | - |
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine | product inhibition | Weissella viridescens | |
0.248 | - |
Ser-tRNA | versus UDP-acetylmuramoylpentapeptide | Weissella viridescens |