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Literature summary for 2.3.1.B43 extracted from

  • Kumar, S.; Lombard, D.B.
    Generation and purification of catalytically active recombinant sirtuin5 (SIRT5) protein (2016), Methods Mol. Biol., 1436, 241-257 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.B43

Cloned(Commentary)

Cloned (Comment) Organism
gene SIRT5, alternative splicing of the human SIRT5 mRNA results in two distinct SIRT5 protein isoforms, differing at their C-termini, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3, method, overview) Homo sapiens

Protein Variants

Protein Variants Comment Organism
H158Y site-directed mutagenesis, catalytically inactive mutant, method, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial matrix
-
 Homo sapiens 5759
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NXA8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme activity assay method, overview Homo sapiens ?
-
-

Synonyms

Synonyms Comment Organism
SIRT5
-
 Homo sapiens
sirtuin 5
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens

General Information

General Information Comment Organism
evolution mammals possess seven sirtuin family members (SIRT1-SIRT7), which display diverse subcellular localization patterns, catalytic activities, protein targets, and biological functions Homo sapiens
physiological function sirtuins are NAD+-dependent enzymes that regulate diverse cellular processes, thereby maintaining metabolic homeostasis and genomic integrity.Sirtuin-family deacylases promote health and longevity in mammals. The sirtuin SIRT5 localizes predominantly to the mitochondrial matrix. SIRT5 preferentially removes negatively charged modifications from its target lysines: succinylation, malonylation, and glutarylation. It regulates protein substrates involved in glucose oxidation, ketone body formation, ammonia detoxification, fatty acid oxidation, and ROS management Homo sapiens