Crystallization (Comment) | Organism |
---|---|
crystal structure of Sirt5 in complex with a thioacetyl peptide is obtained, The corresponding acetyl peptide could not be crystallized with Sirt5 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H158Y | mutation significantly increases the Km for desuccinylation | Homo sapiens |
R105M | mutation significantly increases the Km for desuccinylation | Homo sapiens |
Y102F | mutation significantly increases the Km for desuccinylation | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0058 | - |
KQTAR(N6-malonyl)KSTGGWW | pH 7.5, 37°C | Homo sapiens | |
0.0061 | - |
KQTAR(N6-succinyl)KSTGGWW | pH 7.5, 37°C | Homo sapiens | |
0.0087 | - |
SGASE(N6-succinyl)KDIVHSGWW | pH 7.5, 37°C | Homo sapiens | |
0.014 | - |
SGASE(N6-malonyl)KDIVHSGWW | pH 7.5, 37°C | Homo sapiens | |
0.15 | - |
KTRSG(N6-succinyl)KVMRRWW | pH 7.5, 37°C | Homo sapiens | |
0.45 | - |
KTRSG(N6-malonyl)KVMRRWW | pH 7.5, 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + [carbamoyl phosphate synthase 1]-N6-succinyl-L-lysine | Mus musculus | deletion of Sirt5 in mice increases the level of succinylation on carbamoyl phosphate synthase 1 | nicotinamide + [carbamoyl phosphate synthase 1]-L-lysine + 2'-O-succinyl-ADP ribose | - |
? | |
NAD+ + [protein]-N6-malonyl-L-lysine | Homo sapiens | - |
nicotinamide + [protein]-L-lysine + 2'-O-malonyl-ADP-ribose | - |
? | |
NAD+ + [protein]-N6-succinyl-L-lysine | Homo sapiens | protein lysine succinylation may represent a posttranslational modification that can be reversed by Sirt5 in vivo. Lysine succinylation occurs on several mammalian proteins | nicotinamide + [protein]-L-lysine + 2'-O-succinyl-ADP-ribose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NXA8 | - |
- |
Mus musculus | Q8K2C6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | catalytic efficiency (kcat/Km) of Sirt5 with the histone H3K9 acetyl peptide is about 500fold lower than that of Sirt1. Sirtuin 5 has only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr102 and Arg105) that bind to malonylated and succinylated substrates and define the specificity | Homo sapiens | ? | - |
? | |
NAD+ + KQTAR(N6-malonyl)KSTGGWW | histone H3K9 malonyl peptide | Homo sapiens | nicotinamide + KQTARKSTGGWW + 2'-O-malonyl-ADP-ribose | - |
? | |
NAD+ + KQTAR(N6-succinyl)KSTGGWW | histone H3K9 succinyl peptide | Homo sapiens | nicotinamide + KQTARKSTGGWW + 2'-O-succinyl-ADP-ribose | - |
? | |
NAD+ + KTRSG(N6-malonyl)KVMRRWW | - |
Homo sapiens | nicotinamide + KTRSGKVMRRWW + 2'-O-malonyl-ADP-ribose | - |
? | |
NAD+ + KTRSG(N6-succinyl)KVMRRWW | ACS2 Lys628 peptide | Homo sapiens | nicotinamide + KTRSGKVMRRWW + 2'-O-succinyl-ADP-ribose | - |
? | |
NAD+ + SGASE(N6-malonyl)KDIVHSGWW | - |
Homo sapiens | nicotinamide + SGASEKDIVHSGWW + 2'-O-malonyl-ADP-ribose | - |
? | |
NAD+ + SGASE(N6-succinyl)KDIVHSGWW | glutamate dehydrogenase Lys503 peptide | Homo sapiens | nicotinamide + SGASEKDIVHSGWW + 2'-O-succinyl-ADP-ribose | - |
? | |
NAD+ + [carbamoyl phosphate synthase 1]-N6-succinyl-L-lysine | deletion of Sirt5 in mice increases the level of succinylation on carbamoyl phosphate synthase 1 | Mus musculus | nicotinamide + [carbamoyl phosphate synthase 1]-L-lysine + 2'-O-succinyl-ADP ribose | - |
? | |
NAD+ + [protein]-N6-malonyl-L-lysine | - |
Homo sapiens | nicotinamide + [protein]-L-lysine + 2'-O-malonyl-ADP-ribose | - |
? | |
NAD+ + [protein]-N6-succinyl-L-lysine | protein lysine succinylation may represent a posttranslational modification that can be reversed by Sirt5 in vivo. Lysine succinylation occurs on several mammalian proteins | Homo sapiens | nicotinamide + [protein]-L-lysine + 2'-O-succinyl-ADP-ribose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SIRT5 | - |
Homo sapiens |
SIRT5 | - |
Mus musculus |
sirtuin 5 | - |
Homo sapiens |
sirtuin 5 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
SGASE(N6-succinyl)KDIVHSGWW | pH 7.5, 37°C | Homo sapiens | |
0.025 | - |
KQTAR(N6-malonyl)KSTGGWW | pH 7.5, 37°C | Homo sapiens | |
0.028 | - |
SGASE(N6-malonyl)KDIVHSGWW | pH 7.5, 37°C | Homo sapiens | |
0.037 | - |
KQTAR(N6-succinyl)KSTGGWW | pH 7.5, 37°C | Homo sapiens | |
0.079 | - |
KTRSG(N6-succinyl)KVMRRWW | pH 7.5, 37°C | Homo sapiens | |
0.268 | - |
KTRSG(N6-malonyl)KVMRRWW | pH 7.5, 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of Sirt5 in mice increases the level of succinylation on carbamoyl phosphate synthase 1 | Mus musculus |
physiological function | protein lysine succinylation may represent a posttranslational modification that can be reversed by Sirt5 | Mus musculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
KTRSG(N6-succinyl)KVMRRWW | pH 7.5, 37°C | Homo sapiens | |
0.6 | - |
KTRSG(N6-malonyl)KVMRRWW | pH 7.5, 37°C | Homo sapiens | |
1.6 | - |
SGASE(N6-succinyl)KDIVHSGWW | pH 7.5, 37°C | Homo sapiens | |
2 | - |
SGASE(N6-malonyl)KDIVHSGWW | pH 7.5, 37°C | Homo sapiens | |
4.3 | - |
KQTAR(N6-malonyl)KSTGGWW | pH 7.5, 37°C | Homo sapiens | |
6.1 | - |
KQTAR(N6-succinyl)KSTGGWW | pH 7.5, 37°C | Homo sapiens |