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Literature summary for 2.3.1.B43 extracted from

  • Fischer, F.; Gertz, M.; Suenkel, B.; Lakshminarasimhan, M.; Schutkowski, M.; Steegborn, C.
    Sirt5 deacylation activities show differential sensitivities to nicotinamide inhibition (2012), PLoS One, 7, e45098.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

Protein Variants

Protein Variants Comment Organism
R105L mutant retains wild-type deacetylation activity and partially gains Sirt3-like nicotinamide sensitivity (IC50: 0.189 mM) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
nicotinamide nicotinamide inhibition of Sirt5-dependent deacetylation features weak substrate-dependence but requires high, nonphysiological nicotinamide concentrations. The insensitivity of Sirt5 to physiological nicotinamide concentrations is due to a lack of non-competitive inhibition via base exchange, and the inhibition observed at very high nicotinamide concentrations is likely caused by competition with NAD+ Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NXA8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + benzoyl-RGVL(N6-succinyl)KEYGV-amide carbamoyl phosphate synthetase 1 Lys527 peptide Homo sapiens nicotinamide + benzoyl-RGVLKEYGV-amide + 2'-O-acetyl-ADP-ribose
-
?
NAD+ + FKRGVL(N6-acetyl)KEYGVKV carbamoyl phosphate synthetase 1 Lys527 peptide Homo sapiens nicotinamide + FKRGVLKEYGVKV + 2'-O-acetyl-ADP-ribose
-
?
NAD+ + TRSG(N6-acetyl)KVMR peptide based on an acetyl-CoA synthetase 2 acetylation site Homo sapiens nicotinamide + TRSGKVMR + 2'-O-acetyl-ADP-ribose
-
?

Synonyms

Synonyms Comment Organism
SIRT5
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.021
-
pH 7.8, 37°C, substrate: benzoyl-RGVL(succK)EYGV-amide Homo sapiens nicotinamide
0.7
-
pH 7.8, 37°C, substrate: TRSG(acK)VMR Homo sapiens nicotinamide
1.6
-
pH 7.8, 37°C, substrate: FKRGVL(acK)EYGVKV Homo sapiens nicotinamide