Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R105L | mutant retains wild-type deacetylation activity and partially gains Sirt3-like nicotinamide sensitivity (IC50: 0.189 mM) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
nicotinamide | nicotinamide inhibition of Sirt5-dependent deacetylation features weak substrate-dependence but requires high, nonphysiological nicotinamide concentrations. The insensitivity of Sirt5 to physiological nicotinamide concentrations is due to a lack of non-competitive inhibition via base exchange, and the inhibition observed at very high nicotinamide concentrations is likely caused by competition with NAD+ | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NXA8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + benzoyl-RGVL(N6-succinyl)KEYGV-amide | carbamoyl phosphate synthetase 1 Lys527 peptide | Homo sapiens | nicotinamide + benzoyl-RGVLKEYGV-amide + 2'-O-acetyl-ADP-ribose | - |
? | |
NAD+ + FKRGVL(N6-acetyl)KEYGVKV | carbamoyl phosphate synthetase 1 Lys527 peptide | Homo sapiens | nicotinamide + FKRGVLKEYGVKV + 2'-O-acetyl-ADP-ribose | - |
? | |
NAD+ + TRSG(N6-acetyl)KVMR | peptide based on an acetyl-CoA synthetase 2 acetylation site | Homo sapiens | nicotinamide + TRSGKVMR + 2'-O-acetyl-ADP-ribose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SIRT5 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.021 | - |
pH 7.8, 37°C, substrate: benzoyl-RGVL(succK)EYGV-amide | Homo sapiens | nicotinamide | |
0.7 | - |
pH 7.8, 37°C, substrate: TRSG(acK)VMR | Homo sapiens | nicotinamide | |
1.6 | - |
pH 7.8, 37°C, substrate: FKRGVL(acK)EYGVKV | Homo sapiens | nicotinamide |