BRENDA - Enzyme Database
show all sequences of 2.3.1.B43

Sirt5 deacylation activities show differential sensitivities to nicotinamide inhibition

Fischer, F.; Gertz, M.; Suenkel, B.; Lakshminarasimhan, M.; Schutkowski, M.; Steegborn, C.; PLoS One 7, e45098 (2012)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
-
Homo sapiens
Engineering
Protein Variants
Commentary
Organism
R105L
mutant retains wild-type deacetylation activity and partially gains Sirt3-like nicotinamide sensitivity (IC50: 0.189 mM)
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
nicotinamide
nicotinamide inhibition of Sirt5-dependent deacetylation features weak substrate-dependence but requires high, nonphysiological nicotinamide concentrations. The insensitivity of Sirt5 to physiological nicotinamide concentrations is due to a lack of non-competitive inhibition via base exchange, and the inhibition observed at very high nicotinamide concentrations is likely caused by competition with NAD+
Homo sapiens
Organism
Organism
UniProt
Commentary
Textmining
Homo sapiens
Q9NXA8
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
NAD+ + benzoyl-RGVL(N6-succinyl)KEYGV-amide
carbamoyl phosphate synthetase 1 Lys527 peptide
730706
Homo sapiens
nicotinamide + benzoyl-RGVLKEYGV-amide + 2'-O-acetyl-ADP-ribose
-
-
-
?
NAD+ + FKRGVL(N6-acetyl)KEYGVKV
carbamoyl phosphate synthetase 1 Lys527 peptide
730706
Homo sapiens
nicotinamide + FKRGVLKEYGVKV + 2'-O-acetyl-ADP-ribose
-
-
-
?
NAD+ + TRSG(N6-acetyl)KVMR
peptide based on an acetyl-CoA synthetase 2 acetylation site
730706
Homo sapiens
nicotinamide + TRSGKVMR + 2'-O-acetyl-ADP-ribose
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
SIRT5
-
Homo sapiens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Homo sapiens
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.021
-
pH 7.8, 37°C, substrate: benzoyl-RGVL(succK)EYGV-amide
Homo sapiens
nicotinamide
0.7
-
pH 7.8, 37°C, substrate: TRSG(acK)VMR
Homo sapiens
nicotinamide
1.6
-
pH 7.8, 37°C, substrate: FKRGVL(acK)EYGVKV
Homo sapiens
nicotinamide
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Engineering (protein specific)
Protein Variants
Commentary
Organism
R105L
mutant retains wild-type deacetylation activity and partially gains Sirt3-like nicotinamide sensitivity (IC50: 0.189 mM)
Homo sapiens
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.021
-
pH 7.8, 37°C, substrate: benzoyl-RGVL(succK)EYGV-amide
Homo sapiens
nicotinamide
0.7
-
pH 7.8, 37°C, substrate: TRSG(acK)VMR
Homo sapiens
nicotinamide
1.6
-
pH 7.8, 37°C, substrate: FKRGVL(acK)EYGVKV
Homo sapiens
nicotinamide
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
nicotinamide
nicotinamide inhibition of Sirt5-dependent deacetylation features weak substrate-dependence but requires high, nonphysiological nicotinamide concentrations. The insensitivity of Sirt5 to physiological nicotinamide concentrations is due to a lack of non-competitive inhibition via base exchange, and the inhibition observed at very high nicotinamide concentrations is likely caused by competition with NAD+
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
NAD+ + benzoyl-RGVL(N6-succinyl)KEYGV-amide
carbamoyl phosphate synthetase 1 Lys527 peptide
730706
Homo sapiens
nicotinamide + benzoyl-RGVLKEYGV-amide + 2'-O-acetyl-ADP-ribose
-
-
-
?
NAD+ + FKRGVL(N6-acetyl)KEYGVKV
carbamoyl phosphate synthetase 1 Lys527 peptide
730706
Homo sapiens
nicotinamide + FKRGVLKEYGVKV + 2'-O-acetyl-ADP-ribose
-
-
-
?
NAD+ + TRSG(N6-acetyl)KVMR
peptide based on an acetyl-CoA synthetase 2 acetylation site
730706
Homo sapiens
nicotinamide + TRSGKVMR + 2'-O-acetyl-ADP-ribose
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Homo sapiens
Other publictions for EC 2.3.1.B43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Hang
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-
-
1
1
-
-
-
16
3
-
-
2
-
3
-
-
1
-
-
-
-
-
20
-
2
1
-
-
16
1
-
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1
-
-
-
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-
1
1
1
-
-
-
-
-
16
3
-
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2
-
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1
-
-
-
-
20
-
1
-
-
16
1
-
-
-
-
2
2
-
16
16
756555
Shuai
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68
1449-1461
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-
-
1
-
1
-
-
-
1
-
-
4
-
3
-
-
-
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2
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4
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1
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1
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1
1
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1
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1
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4
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2
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-
4
-
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-
-
-
-
-
-
-
1
4
4
1
-
-
758247
Greene
SIRT5 stabilizes mitochondria ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
116
26625-26632
2019
-
1
1
-
1
-
-
-
1
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2
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3
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2
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3
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2
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1
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1
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2
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2
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-
3
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-
-
-
-
-
-
-
-
1
2
2
1
-
-
755970
Du
Tissue distribution, subcellu ...
Homo sapiens
Biochem. Biophys. Res. Commun.
503
763-769
2018
-
-
1
-
-
-
-
-
2
1
-
-
-
4
-
-
1
-
-
40
-
-
1
2
5
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
1
-
-
-
-
-
1
-
40
-
-
1
2
1
-
-
-
1
-
-
-
-
2
2
-
-
-
756427
Liu
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Homo sapiens
Chem. Biol. Drug Des.
91
257-268
2018
-
-
1
-
-
-
38
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
2
1
-
-
-
1
-
-
1
-
-
1
-
-
1
1
-
-
-
1
40
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
756522
Kumar
Functions of the sirtuin deac ...
Mus musculus, Homo sapiens
Crit. Rev. Biochem. Mol. Biol.
53
311-334
2018
2
1
1
-
-
-
4
-
10
-
-
2
-
4
-
-
-
-
-
14
-
-
2
-
4
-
-
-
-
-
-
-
2
-
-
-
2
1
1
2
-
-
-
-
4
-
-
10
-
-
2
-
-
-
-
-
14
-
-
2
-
-
-
-
-
-
-
-
-
4
8
8
4
-
-
756563
Du
SIRT5 deacylates metabolism-r ...
Mus musculus, Mus musculus C57BL/6
EBioMedicine
36
347-357
2018
-
-
1
-
1
-
-
-
1
-
-
-
-
7
-
-
-
-
-
4
-
-
2
-
1
-
-
-
-
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-
1
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-
1
1
-
1
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1
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4
-
-
2
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
756680
Ma
SIRT5 regulates pancreatic be ...
Homo sapiens
Exp. Therap. Med.
16
1417-1425
2018
-
-
-
-
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
3
-
-
1
-
2
-
-
-
-
-
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-
1
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-
1
-
1
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1
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1
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3
-
-
1
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
757400
Kalbas
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Danio rerio, Homo sapiens
J. Med. Chem.
61
2460-2471
2018
-
-
2
1
-
-
90
-
2
-
-
-
-
4
-
-
2
-
-
-
-
-
1
-
7
1
-
-
-
1
-
-
2
1
-
86
-
-
2
2
1
-
-
86
90
1
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
755788
Rajabi
Mechanism-based inhibitors of ...
Danio rerio, Homo sapiens
Angew. Chem. Int. Ed. Engl.
56
14836-14841
2017
-
-
-
-
-
-
49
-
2
-
-
2
-
7
-
-
-
-
-
-
-
-
2
-
9
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
49
-
-
2
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
756393
Wang
SIRT5 desuccinylates and acti ...
Mus musculus
Cell Rep.
19
2331-2344
2017
-
-
1
-
1
-
-
-
1
-
-
1
-
4
-
-
-
-
-
1
-
-
2
-
1
-
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1
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1
1
-
1
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1
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1
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1
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
757084
Qin
NAD+ dependent deacetylase Si ...
Mus musculus
J. Autoimmun.
81
120-129
2017
-
-
1
-
2
-
-
-
2
-
-
-
-
1
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3
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2
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1
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1
1
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2
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2
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3
-
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-
-
-
-
-
-
-
-
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-
1
3
3
1
-
-
757175
Zhang
Lysine desuccinylase SIRT5 bi ...
Mus musculus, Homo sapiens
J. Biol. Chem.
292
10239-10249
2017
2
-
-
-
2
-
-
-
4
-
-
4
-
9
-
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2
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4
-
4
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2
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2
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2
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2
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4
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4
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2
-
-
4
-
-
-
-
-
-
-
-
-
-
6
6
-
-
-
757184
Hershberger
Sirtuin 5 is required for mou ...
Mus musculus
J. Biol. Chem.
292
19767-19781
2017
-
-
-
-
1
-
-
-
1
-
-
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-
3
-
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2
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3
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1
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1
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1
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1
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2
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2
2
-
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-
757350
Yang
-
Design and synthesis of new 9 ...
Homo sapiens
J. Heterocycl. Chem.
54
1457-1466
2017
-
1
-
-
-
-
22
-
1
-
-
-
-
1
-
-
-
-
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2
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1
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5
22
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1
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2
2
-
-
-
738110
Zhou
SIRT5 promotes IDH2 desuccinyl ...
Mus musculus
EMBO Rep.
17
811-822
2016
-
-
-
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-
-
-
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2
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1
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1
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1
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1
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1
1
1
1
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739445
Yu
Role of the substrate specific ...
Homo sapiens
PLoS ONE
11
e0152467
2016
-
-
1
-
3
-
3
9
-
-
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2
-
3
-
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1
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4
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1
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1
8
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1
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2
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1
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3
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2
3
1
9
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2
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1
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4
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1
8
-
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-
-
-
-
-
-
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-
757590
Kumar
Generation and purification o ...
Homo sapiens
Methods Mol. Biol.
1436
241-257
2016
-
-
1
-
1
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-
-
1
-
-
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4
-
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1
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1
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2
1
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1
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1
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-
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1
1
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1
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1
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1
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-
1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
730347
AbouElfetouh
The E. coli sirtuin CobB shows ...
Escherichia coli
Microbiologyopen
4
66-83
2015
-
-
1
-
-
-
-
-
-
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1
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1
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1
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2
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1
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1
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1
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1
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2
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-
-
-
-
-
-
-
-
1
1
-
-
-
738009
Wang
SIRT5 prevents cigarette smoke ...
Homo sapiens
Cell Stress Chaperones
20
805-810
2015
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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-
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2
-
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1
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3
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1
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2
-
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1
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
738843
Roessler
A novel continuous assay for t ...
Homo sapiens
J. Med. Chem.
58
7217-7223
2015
-
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1
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1
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1
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1
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1
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739069
Nishida
SIRT5 regulates both cytosolic ...
Mus musculus
Mol. Cell
59
321-332
2015
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3
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728962
Roessler
Chemical probing of the human ...
Homo sapiens
Angew. Chem. Int. Ed. Engl.
53
10728-10732
2014
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7
8
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7
7
8
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8
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1
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8
1
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8
8
729547
Tan
Lysine glutarylation is a prot ...
Homo sapiens
Cell Metab.
19
605-617
2014
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1
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729700
Buler
SIRT5 is under the control of ...
Mus musculus
FASEB J.
28
3225-3237
2014
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5
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1
2
2
1
-
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730049
Baeza
Stoichiometry of site-specific ...
Escherichia coli
J. Biol. Chem.
289
21326-21338
2014
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1
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1
1
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-
-
730474
Lutz
Distinct patterns of sirtuin e ...
Homo sapiens
Neuromolecular Med.
16
405-414
2014
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1
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1
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1
-
-
730900
Ringel
Alternate deacylating specific ...
Escherichia coli, Archaeoglobus fulgidus
Protein Sci.
23
1686-1697
2014
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1
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4
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2
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8
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2
2
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2
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1
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2
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8
-
2
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-
2
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-
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-
730922
Pacella-Ince
Mitochondrial SIRT5 is present ...
Homo sapiens
Reprod. Fertil. Dev.
26
1072-1083
2014
-
1
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1
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1
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-
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-
-
1
1
1
1
-
-
731010
Lu
SIRT5 facilitates cancer cell ...
Homo sapiens
Tumour Biol.
35
10699-10705
2014
-
1
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1
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3
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1
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1
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1
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1
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-
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2
2
-
-
-
729138
Lin
SIRT5 desuccinylates and activ ...
Homo sapiens
Biochem. Biophys. Res. Commun.
441
191-195
2013
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2
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-
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-
-
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-
729556
Liu
SIRT5: a safeguard against oxi ...
Rattus norvegicus
Cell. Physiol. Biochem.
32
1050-1059
2013
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1
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1
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1
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1
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1
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1
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1
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1
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-
-
-
-
-
-
-
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-
1
2
2
1
-
-
729708
Zhang
Reversibly acetylated lysine r ...
Escherichia coli, Escherichia coli AD494
FEBS J.
280
1966-1979
2013
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1
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1
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4
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1
1
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1
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1
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2
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1
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-
4
-
1
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-
-
1
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-
-
-
-
-
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-
-
729931
Hu
Acetylation of the response re ...
Escherichia coli
J. Bacteriol.
195
4174-4186
2013
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1
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2
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1
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1
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-
2
-
-
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-
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2
2
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-
730024
Feldman
Activation of the protein deac ...
Homo sapiens
J. Biol. Chem.
288
31350-31356
2013
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9
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2
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1
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-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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730384
Colak
Identification of lysine succi ...
Escherichia coli
Mol. Cell. Proteomics
12
3509-3520
2013
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1
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2
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4
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1
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3
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1
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4
1
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1
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2
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4
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1
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-
3
-
1
-
-
4
1
-
-
-
-
1
1
-
6
6
730390
Park
SIRT5-mediated lysine desuccin ...
Mus musculus
Mol. Cell.
50
919-930
2013
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-
-
-
-
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2
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2
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1
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4
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1
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2
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2
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-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
728857
Maurer
Inhibitors of the NAD(+)-depen ...
Homo sapiens
ACS Med. Chem. Lett.
3
1050-1053
2012
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-
-
-
-
-
20
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1
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1
-
1
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19
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19
20
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729372
Suenkel
Inhibition of the human deacyl ...
Homo sapiens
Bioorg. Med. Chem. Lett.
23
143-146
2012
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2
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3
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2
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3
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3
2
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-
3
-
-
-
-
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-
-
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729732
Nakamura
SIRT5 deacetylates and activat ...
Mus musculus
FEBS Lett.
586
4076-4081
2012
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3
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6
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2
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1
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3
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3
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2
-
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-
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729885
He
Thiosuccinyl peptides as Sirt5 ...
Homo sapiens
J. Am. Chem. Soc.
134
1922-1925
2012
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8
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8
10
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1
-
-
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-
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729993
Zhou
The bicyclic intermediate stru ...
Homo sapiens
J. Biol. Chem.
287
28307-28314
2012
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1
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2
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1
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1
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-
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-
-
-
-
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730161
Madsen
Substrates for efficient fluor ...
Homo sapiens
J. Med. Chem.
55
5582-5590
2012
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1
3
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5
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1
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-
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2
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-
-
-
-
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-
1
-
3
-
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-
-
-
5
-
-
-
-
2
-
-
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2
2
730245
Szczepankiewicz
Synthesis of carba-NAD and the ...
Homo sapiens
J. Org. Chem.
77
7319-7329
2012
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1
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1
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1
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-
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-
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-
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-
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-
1
-
1
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-
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-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730706
Fischer
Sirt5 deacylation activities s ...
Homo sapiens
PLoS One
7
e45098
2012
-
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1
-
1
-
1
-
-
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3
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1
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3
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1
1
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1
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3
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1
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1
-
3
1
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-
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-
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1
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
730713
Gertz
A molecular mechanism for dire ...
Homo sapiens
PLoS One
7
e49761
2012
2
-
1
1
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-
1
-
-
-
-
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3
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1
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1
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-
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2
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1
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1
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
729801
Matsushita
Distinct regulation of mitocho ...
Homo sapiens
Genes Cells
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190-202
2011
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-
-
-
1
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-
3
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-
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5
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-
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-
3
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-
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1
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3
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
1
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730103
Geng
SIRT1 and SIRT5 activity expre ...
Rattus norvegicus
J. Cell. Biochem.
112
3755-3761
2011
-
-
-
-
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2
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3
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4
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1
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2
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4
-
-
-
-
-
-
-
-
-
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1
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1
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730383
Peng
The first identification of ly ...
Mus musculus
Mol. Cell. Proteomics
10
M111.012658
2011
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1
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11
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1
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2
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11
-
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-
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-
730408
Lima
Involvement of protein acetyla ...
Escherichia coli
Mol. Microbiol.
81
1190-1204
2011
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1
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1
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1
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1
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1
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-
-
-
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2
2
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-
-
730964
Du
Sirt5 is a NAD-dependent prote ...
Mus musculus, Homo sapiens
Science
334
806-809
2011
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3
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10
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1
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6
1
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1
3
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6
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3
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10
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1
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6
1
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