Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.B41 extracted from

  • Sun, S.; Liu, Z.; Feng, Y.; Shi, L.; Cao, X.; Cai, Y.; Liu, B.
    Sirt6 deacetylase activity regulates circadian rhythms via Per2 (2019), Biochem. Biophys. Res. Commun., 511, 234-238 .
    View publication on PubMed
Search for more literature for 2.3.1.B41

Cloned(Commentary)

Cloned (Comment) Organism
gene SIRT6, recombinant expression of FLAG-tagged SIRT6 in HEK293 cells, co-expression of Myc-tagged PER2, recombinant expression of FLAG-tagged SIRT6 mutant H133Y in HEK-293 cells, quantitative RT-PCR enzyme expressin analysis Mus musculus

Protein Variants

Protein Variants Comment Organism
H133Y a catalytically inactive mutant of SIRT6, the acetylation level of PER2 is significantly increased in SIRT6-KO HEK293 cells compared to wild-type Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus SIRT6 and Per2 co-localize in the nucleus Mus musculus 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + [Per2]-N6-acetyl-L-lysine Mus musculus
-
 nicotinamide + [Per2]-L-lysine + 2'-O-acetyl-ADP ribose
-
 ?
NAD+ + [protein]-N6-palmitoyl-L-lysine Mus musculus
-
 nicotinamide + [protein]-L-lysine + 2'-O-palmitoyl-ADP ribose
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus P59941
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant FLAG-tagged SIRT6 and Myc-tagged PER2 co-purify from HEK293 cells Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
embryonic fibroblast MEF cells Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + [Per2]-N6-acetyl-L-lysine
-
 Mus musculus nicotinamide + [Per2]-L-lysine + 2'-O-acetyl-ADP ribose
-
 ?
NAD+ + [protein]-N6-palmitoyl-L-lysine
-
 Mus musculus nicotinamide + [protein]-L-lysine + 2'-O-palmitoyl-ADP ribose
-
 ?

Synonyms

Synonyms Comment Organism
Sirt6 deacetylase
-
 Mus musculus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Mus musculus

General Information

General Information Comment Organism
malfunction Loss of Sirt6 jeopardizes circadian phase. Reduction of Per2 protein level in Sirt6 null cells Mus musculus
metabolism circadian clock relies on a transcription and translation feedback loop (TTFL). Two transcription factors, i.e. Bmal1 and Clock, activate the transcription of Period (Per) and Cryptochrome (Cry), which inhibit their own transcription when accumulated to a critical concentration. NAD+-dependent deacylase Sirt1 deacetylates Bmal1 and Per2 to regulate circadian rhythms. Sirt6 interacts with Bmal1 to regulate clock-controlled gene (CCG) expression by local chromatin remodeling. Loss of Sirt6 jeopardizes circadian phase. At molecular level, Sirt6 interacts with and deacetylates Per2, thus preventing its proteasomal degradation. Important function of Sirt6 in the direct regulation of TTFL and circadian rhythms Mus musculus
physiological function Sirt6 deacetylase activity regulates circadian rhythms via Per2. Sirt6 interacts with and deacetylates Per2, thus preventing its proteasomal degradation. SIRT6 regulates PER2 protein stability via the ubiquitin-proteasome pathway. Important function of Sirt6 in the direct regulation of TTFL and circadian rhythms. The deacetylase activity of SIRT6 is required for PER2 deacetylation Mus musculus