Crystallization (Comment) | Organism |
---|---|
vapor diffusion at 20°C, crystal structure of the enzyme bound to KGLGKGGA(N6-myristoyl)KRHRKW | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O30124 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with KGLGKGGA(N6-succinyl)KRHRKW | Archaeoglobus fulgidus | ? | - |
? | |
NAD+ + KGLGKGGA(N6-acetyl)KRHRKW | the enzyme shows increased reaction velocity with increasing acyl chain length. As compared to its deacetylating activity, Sir2Af2 depropionylates, debutyrylates, and demyristoylates a peptide of the same sequence at 1.5-, 1.9-, and 3.4-fold higher rates, respectively | Archaeoglobus fulgidus | nicotinamide + KGLGKGGAKRHRKW + 2'-O-acetyl-ADP-ribose | - |
? | |
NAD+ + KGLGKGGA(N6-butyryl)KRHRKW | the enzyme shows increased reaction velocity with increasing acyl chain length. As compared to its deacetylating activity, Sir2Af2 depropionylates, debutyrylates, and demyristoylates a peptide of the same sequence at 1.5-, 1.9-, and 3.4-fold higher rates, respectively | Archaeoglobus fulgidus | nicotinamide + KGLGKGGAKRHRKW + 2'-O-butyryl-ADP-ribose | - |
? | |
NAD+ + KGLGKGGA(N6-myristoyl)KRHRKW | the enzyme shows increased reaction velocity with increasing acyl chain length. As compared to its deacetylating activity, Sir2Af2 depropionylates, debutyrylates, and demyristoylates a peptide of the same sequence at 1.5-, 1.9-, and 3.4-fold higher rates, respectively | Archaeoglobus fulgidus | nicotinamide + KGLGKGGAKRHRKW + 2'-O-myristoyl-ADP-ribose | - |
? | |
NAD+ + KGLGKGGA(N6-propionyl)KRHRKW | the enzyme shows increased reaction velocity with increasing acyl chain length. As compared to its deacetylating activity, Sir2Af2 depropionylates, debutyrylates, and demyristoylates a peptide of the same sequence at 1.5-, 1.9-, and 3.4-fold higher rates, respectively | Archaeoglobus fulgidus | nicotinamide + KGLGKGGAKRHRKW + 2'-O-propionyl-ADP-ribose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Sir2Af2 | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Archaeoglobus fulgidus |