BRENDA - Enzyme Database
show all sequences of 2.3.1.B33

Dual catalytic activity of hydroxycinnamoyl-coenzyme A quinate transferase from tomato allows it to moonlight in the synthesis of both mono- and dicaffeoylquinic acids

Moglia, A.; Lanteri, S.; Comino, C.; Hill, L.; Knevitt, D.; Cagliero, C.; Rubiolo, P.; Bornemann, S.; Martin, C.; Plant Physiol. 166, 1777-1787 (2014)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dithiothreitol
slight increase in activity
Solanum lycopersicum
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Solanum lycopersicum
Crystallization (Commentary)
Crystallization
Organism
homology modeling based on structure 4KEC of Sorghum bicolor hydroxycinnamoyl-coenzyme A transferase
Solanum lycopersicum
Engineering
Amino acid exchange
Commentary
Organism
H276Y
58% of wild-type activity for production of dicaffeoylquinic acids, 92% of wild-type hydroxycinnamoyltranferase activity
Solanum lycopersicum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
19
-
chlorogenic acid
pH 4.0, 30°C
Solanum lycopersicum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
enzyme catalyzes different reactions in different compartments
Solanum lycopersicum
5737
-
vacuole
enzyme catalyzes different reactions in different compartments
Solanum lycopersicum
5773
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
slight increase in activity
Solanum lycopersicum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 chlorogenic acid
Solanum lycopersicum
reaction is irreversible
1,5-dicaffeoylquinic acid + quinate
products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid
-
ir
2 chlorogenic acid
Solanum lycopersicum
reaction is irreversible
3,5-dicaffeoylquinic acid + quinate
products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid
-
ir
2 chlorogenic acid
Solanum lycopersicum
reaction is irreversible
4,5-dicaffeoylquinic acid + quinate
products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Solanum lycopersicum
Q70G32
bifunctional enzyme, displays hydroxycinnamoyl-Coenzyme A:quinate hydroxycinnamoyl transferase activity and chlorogenate:chlorogenate transferase activity
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
increase in CCT activity is observed during ripening, reaching a maximum in the peel of red fruit
Solanum lycopersicum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
22.3
-
pH 4.0, 30°C
Solanum lycopersicum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 chlorogenic acid
reaction is irreversible
737028
Solanum lycopersicum
1,5-dicaffeoylquinic acid + quinate
products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid
-
-
ir
2 chlorogenic acid
reaction is irreversible
737028
Solanum lycopersicum
3,5-dicaffeoylquinic acid + quinate
products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid
-
-
ir
2 chlorogenic acid
reaction is irreversible
737028
Solanum lycopersicum
4,5-dicaffeoylquinic acid + quinate
products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid
-
-
ir
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4
-
-
Solanum lycopersicum
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactor required
Solanum lycopersicum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dithiothreitol
slight increase in activity
Solanum lycopersicum
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Solanum lycopersicum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactor required
Solanum lycopersicum
Crystallization (Commentary) (protein specific)
Crystallization
Organism
homology modeling based on structure 4KEC of Sorghum bicolor hydroxycinnamoyl-coenzyme A transferase
Solanum lycopersicum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
H276Y
58% of wild-type activity for production of dicaffeoylquinic acids, 92% of wild-type hydroxycinnamoyltranferase activity
Solanum lycopersicum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
19
-
chlorogenic acid
pH 4.0, 30°C
Solanum lycopersicum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
enzyme catalyzes different reactions in different compartments
Solanum lycopersicum
5737
-
vacuole
enzyme catalyzes different reactions in different compartments
Solanum lycopersicum
5773
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
slight increase in activity
Solanum lycopersicum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 chlorogenic acid
Solanum lycopersicum
reaction is irreversible
1,5-dicaffeoylquinic acid + quinate
products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid
-
ir
2 chlorogenic acid
Solanum lycopersicum
reaction is irreversible
3,5-dicaffeoylquinic acid + quinate
products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid
-
ir
2 chlorogenic acid
Solanum lycopersicum
reaction is irreversible
4,5-dicaffeoylquinic acid + quinate
products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid
-
ir
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
increase in CCT activity is observed during ripening, reaching a maximum in the peel of red fruit
Solanum lycopersicum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
22.3
-
pH 4.0, 30°C
Solanum lycopersicum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 chlorogenic acid
reaction is irreversible
737028
Solanum lycopersicum
1,5-dicaffeoylquinic acid + quinate
products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid
-
-
ir
2 chlorogenic acid
reaction is irreversible
737028
Solanum lycopersicum
3,5-dicaffeoylquinic acid + quinate
products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid
-
-
ir
2 chlorogenic acid
reaction is irreversible
737028
Solanum lycopersicum
4,5-dicaffeoylquinic acid + quinate
products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid
-
-
ir
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4
-
-
Solanum lycopersicum
Other publictions for EC 2.3.1.B33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737028
Moglia
Dual catalytic activity of hyd ...
Solanum lycopersicum
Plant Physiol.
166
1777-1787
2014
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