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Literature summary for 2.3.1.99 extracted from

  • Pu, G.; Zhou, B.; Xiang, F.
    Isolation and functional characterization of a Lonicera japonica hydroxycinnamoyl transferase involved in chlorogenic acid synthesis (2017), Biologia (Poland), 72, 608-618 .
No PubMed abstract available
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.077
-
 quinate cosubstrate 4-coumaroyl-CoA, pH not specified in the publication, 30°C Lonicera japonica
0.146
-
 5-O-caffeoylquinate cosubstrate CoA, pH not specified in the publication, 30°C Lonicera japonica
0.203
-
 quinate cosubstrate caffeoyl-CoA, pH not specified in the publication, 30°C Lonicera japonica
0.519
-
 4-coumaroyl-CoA cosubstrate quinate, pH not specified in the publication, 30°C Lonicera japonica
0.914
-
 caffeoyl-CoA cosubstrate quinate, pH not specified in the publication, 30°C Lonicera japonica
5.579
-
 shikimate cosubstrate 4-coumaroyl-CoA, pH not specified in the publication, 30°C Lonicera japonica

Organism

Organism UniProt Comment Textmining
Lonicera japonica G0ZDQ5
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Lonicera japonica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + quinate
-
 Lonicera japonica CoA + 5-O-(4-coumaroyl)quinate
-
 ?
4-coumaroyl-CoA + shikimate
-
 Lonicera japonica CoA + 5-O-(4-coumaroyl)shikimate
-
 ?
caffeoyl-CoA + quinate
-
 Lonicera japonica CoA + 5-O-caffeoylquinate
-
 ?
CoA + 5-O-caffeoylquinate
-
 Lonicera japonica caffeoyl-CoA + quinate
-
 ?

Synonyms

Synonyms Comment Organism
HQT
-
 Lonicera japonica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 shikimate cosubstrate 4-coumaroyl-CoA, pH not specified in the publication, 30°C Lonicera japonica
0.9
-
 5-O-caffeoylquinate cosubstrate CoA, pH not specified in the publication, 30°C Lonicera japonica
1.3
-
 caffeoyl-CoA cosubstrate quinate, pH not specified in the publication, 30°C Lonicera japonica
1.65
-
 quinate cosubstrate caffeoyl-CoA, pH not specified in the publication, 30°C Lonicera japonica
1.88
-
 4-coumaroyl-CoA cosubstrate quinate, pH not specified in the publication, 30°C Lonicera japonica
2.75
-
 quinate cosubstrate 4-coumaroyl-CoA, pH not specified in the publication, 30°C Lonicera japonica

Expression

Organism Comment Expression
Lonicera japonica expression is induced by salicylic acid, abscisic acid and exposure to low temperature up

General Information

General Information Comment Organism
physiological function constitutive expression of HQT in Arabidopsis thaliana results in the production of 5-caffeoylquinic acid in the leaf and an higher level of resistance to the bacterial pathogen Pseudomonas syringae. 5-caffeoylquinic acid synthesis is positively correlated with HQT transcript abundance Lonicera japonica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.167
-
 caffeoyl-CoA cosubstrate quinate, pH not specified in the publication, 30°C Lonicera japonica
0.9
-
 5-O-caffeoylquinate cosubstrate CoA, pH not specified in the publication, 30°C Lonicera japonica
3.67
-
 4-coumaroyl-CoA cosubstrate quinate, pH not specified in the publication, 30°C Lonicera japonica
6.17
-
 shikimate cosubstrate 4-coumaroyl-CoA, pH not specified in the publication, 30°C Lonicera japonica
8
-
 quinate cosubstrate caffeoyl-CoA, pH not specified in the publication, 30°C Lonicera japonica
35.67
-
 quinate cosubstrate 4-coumaroyl-CoA, pH not specified in the publication, 30°C Lonicera japonica