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Literature summary for 2.3.1.98 extracted from

  • Teutschbein, J.; Gross, W.; Nimtz, M.; Milkowski, C.; Hause, B.; Strack, D.
    Identification and localization of a lipase-like acyltransferase in phenylpropanoid metabolism of tomato (Solanum lycopersicum) (2010), J. Biol. Chem., 285, 38374-38381.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.98

Cloned(Commentary)

Cloned (Comment) Organism
gene SlCGT, functional expression in Nicotiana benthamiana leaves using Agrobacterium tumefaciens strain GV2260 transfection method Solanum lycopersicum

Protein Variants

Protein Variants Comment Organism
D328A site-directed mutagenesis, the mutant shows 40% reduced activity Solanum lycopersicum
S27A site-directed mutagenesis, the mutant shows 13% reduced activity Solanum lycopersicum

Inhibitors

Inhibitors Comment Organism Structure
PMSF treatment of purified SlCGT with 1, 10, and 50 mM PMSF leads to strong decreases in caffeoyl transfer activities to 60%, 40%, and 0% activity, respectively Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
apoplast apoplastic space of tomato leaves Solanum lycopersicum 48046
-
additional information SiCGT contains a putative targeting signal of 24 amino acids indicating an entry of the SlCGT into the secretory pathway Solanum lycopersicum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39950
-
 x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation Solanum lycopersicum
40000
-
 x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation Solanum lycopersicum
42580
-
 x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorogenate + glucarate Solanum lycopersicum the enzyme catalyzes the formation of caffeoylglucarate and caffeoylgalactarate using chlorogenate, i.e. 5-O-caffeoylquinate, as acyl donor quinate + 2-O-caffeoylglucarate
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum E7AIM3 cv. Moneymaker, gene SlCGT, single-copy gene
-

Purification (Commentary)

Purification (Comment) Organism
native SlCGT 585fold from seedlings by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, ultrafiltration, gel filtration, and again anion exchange chromatography Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Solanum lycopersicum
-
fruit at different stages of ripening Solanum lycopersicum
-
leaf apoplastic space of tomato leaves Solanum lycopersicum
-
additional information tissue expression pattern, overview. Transcripts of SlCGT, enzyme activity, and caffeoylglucarate accumulate preferentially in tomato leaves and flowers Solanum lycopersicum
-
root
-
 Solanum lycopersicum
-
seedling
-
 Solanum lycopersicum
-
stem
-
 Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.45
-
 purified native enzyme, pH 6.0, 30°C Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorogenate + glucarate
-
 Solanum lycopersicum quinate + 2-O-caffeoylglucarate
-
 ?
chlorogenate + glucarate the enzyme catalyzes the formation of caffeoylglucarate and caffeoylgalactarate using chlorogenate, i.e. 5-O-caffeoylquinate, as acyl donor Solanum lycopersicum quinate + 2-O-caffeoylglucarate
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation Solanum lycopersicum
More peptide sequences are obtained by trypsin digestion and spectrometric sequencing, structure-function analysis, overview Solanum lycopersicum

Synonyms

Synonyms Comment Organism
CGT
-
 Solanum lycopersicum
chlorogenate:glucarate caffeoyltransferase
-
 Solanum lycopersicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Solanum lycopersicum

pI Value

Organism Comment pI Value Maximum pI Value
Solanum lycopersicum sequence calculation, mature CGT without signal sequence
-
 5.01
Solanum lycopersicum sequence calculation, CGT with signal sequence
-
 5.25

General Information

General Information Comment Organism
evolution the sequence determines SlCGT as a GDSL lipase-like protein, representing it as a member of the SGNH protein superfamily. Lipases of this family employ a catalytic triad of Ser-Asp-His with Ser as nucleophile of the GDSL motif. The catalytic triad of the GDSL lipase is not essential for SlCGT enzymatic activity. SlCGT is therefore an example of a GDSL lipase-like protein that has lost hydrolytic activity and has acquired a completely new function in plant metabolism, functioning in secondary metabolism as acyltransferase in synthesis of hydroxycinnamate esters by employing amino acid residues different from the lipase catalytic triad Solanum lycopersicum
additional information the deduced SlCGT amino acid sequence matches the conservation of the serine catalytic motif GXSXXDXG within the first consensus block near the N-terminus. A serine moiety of the GDSL protein may be part of the catalytic center in SlCGT Solanum lycopersicum