Literature summary for 2.3.1.97 extracted from

Kumar, S.; Sharma, R.
N-terminal region of the catalytic domain of human N-myristoyltransferase 1 acts as an inhibitory module (2015), PLoS ONE, 10, e0127661.

Search for more literature for 2.3.1.97

Cloned(Commentary)

Cloned (Comment)	Organism
-	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	characterziation of a truncation mutant devoid of 28 N-terminal amino acids from the catalytic module. The deletion of the N-terminal peptide has no effect on the enzyme stability. Mutant displays an 3fold increase in enzymatic. The N-terminal region in the catalytic module serves as a regulatory control element	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00057	-	myristoyl-CoA	wild-type, pH 7.5, 25°C	Homo sapiens
0.00104	-	myristoyl-CoA	N-terminal truncation mutant, pH 7.5, 25°C	Homo sapiens
0.0144	-	HIV-1 negative regulatory factor	N-terminal truncation mutant, pH 7.5, 25°C	Homo sapiens
0.0257	-	HIV-1 negative regulatory factor	wild-type, pH 7.5, 25°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P30419	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
myristoyl-CoA + HIV-1 negative regulatory factor	-	Homo sapiens	N-myristoyl-HIV-1 negative regulatory factor + CoA	-	?

Synonyms

Synonyms	Comment	Organism
NMT1	-	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
55.9	-	melting temperature, N-terminal truncation mutant	Homo sapiens
56.4	-	melting temperature, wild-type	Homo sapiens
58.6	-	melting temperature, N-terminal truncation mutant, presence of myristoyl-CoA	Homo sapiens
59.5	-	melting temperature, wild-type, presence of myristoyl-CoA	Homo sapiens

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Release 2023.1 (January 2023)