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Literature summary for 2.3.1.97 extracted from

  • Flamm, A.G.; Le Roux, A.L.; Mateos, B.; Diaz-Lobo, M.; Storch, B.; Breuker, K.; Konrat, R.; Pons, M.; Coudevylle, N.
    N-Lauroylation during the expression of recombinant N-myristoylated proteins: implications and solutions (2016), ChemBioChem, 17, 82-89.
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
synthesis recombinant expression of N-myristoylated proteins in Escherichia coli can be achieved by co-expressing N-myristoyltransferase and supplementing the growth medium with myristic acid. Undesired incorporation of the 12-carbon fatty acid lauric acid can also occur, leading to heterogeneous samples. A strategy for obtaining lauryl-free samples of myristoylated proteins in both rich and minimal media Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lauroyl-CoA + brain acid soluble protein 1
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 Homo sapiens N-lauroyl-brain acid soluble protein 1 + CoA N-laurylation of recombinant proteins in Escherichia coli may occur in minimal media ?
lauroyl-CoA + c-Src N-terminal region of c-Src, residues 1-185 Homo sapiens N-lauroyl-c-Src + CoA N-laurylation of recombinant proteins in Escherichia coli may occur in minimal media ?
myristoyl-CoA + brain acid soluble protein 1
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 Homo sapiens N-myristoyl-brain acid soluble protein 1 + CoA
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 ?
myristoyl-CoA + c-Src N-terminal region of c-Src, residues 1-185 Homo sapiens N-myristoyl-c-Src + CoA
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 ?