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Literature summary for 2.3.1.97 extracted from

  • Roberts, A.J.; Torrie, L.S.; Wyllie, S.; Fairlamb, A.H.
    Biochemical and genetic characterization of Trypanosoma cruzi N-myristoyltransferase (2014), Biochem. J., 459, 323-332.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.97

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
2,6-dichloro-4-[2-(piperazin-1-yl)pyridin-4-yl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)benzenesulfonamide inhibits growth of Trypanosoma cruzi epimastigotes with EC50 of 6.9 microM, but is about 1000fold less potent than in Trypanosma brucei Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0062
-
 myristoyl-CoA pH 7.4, 30°C Trypanosoma cruzi
0.012
-
 CAP5.5 peptide source Trypanosoma cruzi, pH 7.4, 30°C Trypanosoma cruzi
0.25
-
 CAP5.5 peptide source Trypanosoma brucei, pH 7.4, 30°C Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47400
-
 gel filtration Trypanosoma cruzi
53700
-
 1 * 53700, calculated, 1 * 53700, mass spectrometry Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myristoyl-CoA + CAP5.5 peptide
-
 Trypanosoma cruzi N-myristoylglycyl-CAP5.5 peptide + CoA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 53700, calculated, 1 * 53700, mass spectrometry Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 CAP5.5 peptide source Trypanosoma cruzi, pH 7.4, 30°C Trypanosoma cruzi
0.34
-
 myristoyl-CoA pH 7.4, 30°C Trypanosoma cruzi
2.11
-
 CAP5.5 peptide source Trypanosoma brucei, pH 7.4, 30°C Trypanosoma cruzi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000228
-
 2,6-dichloro-4-[2-(piperazin-1-yl)pyridin-4-yl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)benzenesulfonamide pH 7.4, 30°C Trypanosoma cruzi

General Information

General Information Comment Organism
physiological function expression of NMT gene is essential for survival of Trypanosoma cruzi epimastigotes Trypanosoma cruzi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8.44
-
 CAP5.5 peptide source Trypanosoma brucei, pH 7.4, 30°C Trypanosoma cruzi
12.4
-
 CAP5.5 peptide source Trypanosoma cruzi, pH 7.4, 30°C Trypanosoma cruzi
54.8
-
 myristoyl-CoA pH 7.4, 30°C Trypanosoma cruzi