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Literature summary for 2.3.1.97 extracted from

  • Fang, W.; Robinson, D.A.; Raimi, O.G.; Blair, D.E.; Harrison, J.R.; Lockhart, D.E.; Torrie, L.S.; Ruda, G.F.; Wyatt, P.G.; Gilbert, I.H.; van Aalten, D.M.
    N-myristoyltransferase is a cell wall target in Aspergillus fumigatus (2015), ACS Chem. Biol., 10, 1425-1434.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.97

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aspergillus fumigatus

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with inhibitors 2,6-dichloro-4-[2-(4-methylpiperazin-1-yl)pyridin-4-yl]-N-(1,5-dimethyl-3-isobutyl-1H-pyrazol-4-yl)benzenesulfonamide and 2,6-dichloro-4-[2-(4-methylpiperazin-1-yl)pyridin-4-yl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)benzenesulfonamide binding in the peptide substrate pocket Aspergillus fumigatus

Inhibitors

Inhibitors Comment Organism Structure
2,6-dichloro-4-[2-(4-methylpiperazin-1-yl)pyridin-4-yl]-N-(1,5-dimethyl-3-isobutyl-1H-pyrazol-4-yl)benzenesulfonamide inhibits the enzyme and is fungicidal under partially repressive nmt conditions Aspergillus fumigatus
2,6-dichloro-4-[2-(piperazin-1-yl)pyridin-4-yl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)benzenesulfonamide
-
 Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 myristoleoyl-CoA pH not specified in the publication, temperature not specified in the publication Aspergillus fumigatus
0.022
-
 CAP5.5 pH not specified in the publication, temperature not specified in the publication Aspergillus fumigatus

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus Q9UVX3
-
-
Aspergillus fumigatus ATCC MYA-4609 Q9UVX3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myristoleoyl-CoA + CAP5.5 acceptor is biotin-tagged cytoskeleton-associated protein 5.5 Aspergillus fumigatus N-myristoleoyl-CAP5.5 + CoA
-
 ?
myristoleoyl-CoA + CAP5.5 acceptor is biotin-tagged cytoskeleton-associated protein 5.5 Aspergillus fumigatus ATCC MYA-4609 N-myristoleoyl-CAP5.5 + CoA
-
 ?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000012
-
 pH not specified in the publication, temperature not specified in the publication Aspergillus fumigatus 2,6-dichloro-4-[2-(4-methylpiperazin-1-yl)pyridin-4-yl]-N-(1,5-dimethyl-3-isobutyl-1H-pyrazol-4-yl)benzenesulfonamide
0.000023
-
 pH not specified in the publication, temperature not specified in the publication Aspergillus fumigatus 2,6-dichloro-4-[2-(piperazin-1-yl)pyridin-4-yl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)benzenesulfonamide

General Information

General Information Comment Organism
physiological function expression of the Nmt gene is essential for viability. Partial repression of the gene reveals downstream effects of N-myristoylation on cell wall morphology Aspergillus fumigatus