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Literature summary for 2.3.1.97 extracted from
- A continuous assay of myristoyl-CoA:protein N-myristoyltransferase for proteomic analysis (2003), Anal. Biochem., 322, 116-123.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | the automated assay can be used for proteomic studies to determine the myristoylation state of any protein | Saccharomyces cerevisiae |
| analysis | the automated assay can be used for proteomic studies to determine the myristoylation state of any protein | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| palmitoyl-CoA | - |
Arabidopsis thaliana |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Saccharomyces cerevisiae | |
| additional information | - |
additional information | - |
Arabidopsis thaliana | |
| 0.001 | - |
myristoyl-CoA | pH 8.0, 30°C, continuous assay | Saccharomyces cerevisiae | |
| 0.0015 | - |
myristoyl-CoA | pH 8.0, 30°C, continuous assay | Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myristoyl-CoA + glycylpeptide | Saccharomyces cerevisiae | - |
N-myristoylglycylpeptide + CoA | - |
? | |
| myristoyl-CoA + glycylpeptide | Arabidopsis thaliana | - |
N-myristoylglycylpeptide + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9LTR9 | - |
- |
| Saccharomyces cerevisiae | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
continuous assay for proteomic analysis | Saccharomyces cerevisiae |
| additional information | - |
continuous assay for proteomic analysis | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myristoyl-CoA + glycylpeptide | - |
Saccharomyces cerevisiae | N-myristoylglycylpeptide + CoA | - |
? | |
| myristoyl-CoA + glycylpeptide | - |
Arabidopsis thaliana | N-myristoylglycylpeptide + CoA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| myristoyl-CoA:protein N-myristoyltransferase | - |
Saccharomyces cerevisiae |
| myristoyl-CoA:protein N-myristoyltransferase | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Saccharomyces cerevisiae | |
| additional information | - |
additional information | - |
Arabidopsis thaliana | |
| 0.234 | - |
myristoyl-CoA | pH 8.0, 30°C, continuous assay | Arabidopsis thaliana | |
| 0.49 | - |
myristoyl-CoA | pH 8.0, 30°C, continuous assay | Saccharomyces cerevisiae | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Arabidopsis thaliana | |
| 0.0007 | - |
palmitoyl-CoA | substrate AtSOS3 + myristoyl-CoA | Arabidopsis thaliana | |
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Release 2023.1 (January 2023)
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