Literature summary for 2.3.1.97 extracted from

Farazi, T.A.; Waksman, G.; Gordon, J.I.
Structures of Saccharomyces cerevisiae N-myristoyltransferase with bound myristoylCoA and peptide provide insights about substrate recognition and catalysis (2001), Biochemistry, 40, 6335-6343.

Search for more literature for 2.3.1.97

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drops over a solution of 0.1 M ammonium acetate, 0.1 M sodium cacodylate, pH 6.4, 20°C, 20% polyethylene glycol 4000, protein 25 mg/ml, a few days, structure determination and analysis by X-ray diffraction of binary and ternary complexes of enzyme, peptide substrate or myristoyl-CoA and inhibitor	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
S-(2-Oxo)-pentadecyl-CoA	-	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P14743	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]	detailed mechanism	Saccharomyces cerevisiae	a
tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]	peptide binding site	Saccharomyces cerevisiae	a
tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]	myristoyl-CoA binding site	Saccharomyces cerevisiae	a
tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]	binding of myristoyl-CoA to the enzyme occurs through at least a 2-step process, X-ray data structure analysis of a binary complex between enzyme and inhibitor S-(2-oxo)-pentadecyl-CoA and ternary with peptide substrate	Saccharomyces cerevisiae	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
myristoyl-CoA + glycylpeptide	specific for Gly at N-terminus	Saccharomyces cerevisiae	N-myristoylglycylpeptide + CoA	-	?

Synonyms

Synonyms	Comment	Organism
Nmt1p	-	Saccharomyces cerevisiae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)