Literature summary for 2.3.1.97 extracted from

Farazi, T.A.; Manchester, J.K.; Gordon, J.I.
Transient-state kinetic analysis of Saccharomyces cerevisiae myristoylCoA:protein N-myristoyltransferase reveals that a step after chemical transformation is rate limiting (2000), Biochemistry, 39, 15807-15816.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
S-(2-Oxo)-pentadecyl-CoA	-	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P14743	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant from Escherichia coli	Saccharomyces cerevisiae

Reaction

Reaction	Comment	Organism	Reaction ID
tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]	ordered bi bi mechanism	Saccharomyces cerevisiae	a
tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]	kinetic analysis	Saccharomyces cerevisiae	a
tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]	active site	Saccharomyces cerevisiae	a
tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]	binding of myristoyl-CoA to the enzyme occurs through at least a 2-step process, X-ray data structure analysis of a binary complex between enzyme and inhibitor S-(2-oxo)-pentadecyl-CoA and ternary with peptide substrate	Saccharomyces cerevisiae	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
myristoyl-CoA + glycylpeptide	-	Saccharomyces cerevisiae	N-myristoylglycylpeptide + CoA	-	?

Synonyms

Synonyms	Comment	Organism
More	enzyme belongs to the superfamily of GCN5-related N-acetyltransferases	Saccharomyces cerevisiae
Nmt1p	-	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
13.8	-	myristoyl-CoA	chemical transformation step	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)