Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.97 extracted from

  • Towler, D.E.; Adams, S.P.; Eubanks, S.R.; Towery, D.S.; Jackson-Machelski, E.; Glaser, L.; Gordon, J.I.
    Purification and characterization of yeast myristoyl CoA:protein N-myristoyltransferase [published erratum appears in Proc Natl Acad Sci U S A 1987 Nov;84(21):7523] (1987), Proc. Natl. Acad. Sci. USA, 84, 2708-2712.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.97

Activating Compound

Activating Compound Comment Organism Structure
EDTA slight activation Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 0.1 mM, 30% inhibition Saccharomyces cerevisiae
diethyldicarbonate
-
 Saccharomyces cerevisiae
p-hydroxymercuribenzoate
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Saccharomyces cerevisiae
0.016
-
 Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae
0.016
-
 Gly-(diaminobutyrate-gamma-NH2-4-azido-3-iodosalicylamide)-L-Ala-L-Ala-L-Ser-S-Ala-L-Arg-L-Arg
-
 Saccharomyces cerevisiae
0.04
-
 Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys
-
 Saccharomyces cerevisiae
0.06
-
 Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae
0.06
-
 Gly-Asn-Phe-Ala-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae
0.3
-
 Gly-Asn-Ala-Pro-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae
0.3
-
 Gly-Ala-Arg-Ala-Ala-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae
1.3
-
 Gly-Asn-Ala-Ala-Ala-Ala
-
 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for divalent cations Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein] requires alanine at position 5 of substrate peptides Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myristoyl-CoA + Gly-Ala-Arg-Ala-Ala-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae N-myristoyl-Gly-Ala-Arg-Ala-Ala-Ala-Ala-Arg-Arg + CoA
-
 ?
myristoyl-CoA + Gly-Asn-Ala-Ala-Ala-Ala
-
 Saccharomyces cerevisiae N-myristoyl-Gly-Asn-Ala-Ala-Ala-Ala + CoA
-
 ?
myristoyl-CoA + Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae N-myristoyl-Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg + CoA
-
 ?
myristoyl-CoA + Gly-Asn-Ala-Pro-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae N-myristoyl-Gly-Asn-Ala-Pro-Ala-Ala-Arg-Arg + CoA
-
 ?
myristoyl-CoA + Gly-Asn-Phe-Ala-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae N-myristoyl-Gly-Asn-Phe-Ala-Ala-Ala-Arg-Arg + CoA
-
 ?
myristoyl-CoA + Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys
-
 Saccharomyces cerevisiae N-myristoyl-Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys + CoA
-
 ?
myristoyl-CoA + glycylpeptide substrate specificity Saccharomyces cerevisiae N-myristoylglycylpeptide + CoA
-
 ?
myristoyl-CoA + glycylpeptide myristoyl-peptides can compete with myristoyl-CoA for the binding site Saccharomyces cerevisiae N-myristoylglycylpeptide + CoA
-
 ?
myristoyl-CoA + glycylpeptide overview peptides and proteins Saccharomyces cerevisiae N-myristoylglycylpeptide + CoA
-
 ?
myristoyl-CoA + glycylpeptide specific for Gly at N-terminus Saccharomyces cerevisiae N-myristoylglycylpeptide + CoA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
NMT
-
 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
7 9 about half-maximal activity above pH 9.0 and below pH 7.0, inactive at pH 6.0 Saccharomyces cerevisiae