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Literature summary for 2.3.1.95 extracted from

  • Suh, D.Y.; Fukuma, K.; Kagami, J.; Yamazaki, Y.; Shibuya, M.; Ebizuka, Y.; Sankawa, U.
    Identification of amino acid residues important in the cyclization reactions of chalcone and stilbene synthases (2000), Biochem. J., 350, 229-235.
No PubMed abstract available
Search for more literature for 2.3.1.95

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type, P375G and G374L mutant enzyme in Escherichia coli Arachis hypogaea

Protein Variants

Protein Variants Comment Organism
G374L no condensing activity Arachis hypogaea
P375G product profile of p-coumaroyl condensing reaction changed to: 42% p-coumaroyltriacetic acid, 27% resveratrol, 24% bisnoryangonin and 7.3% naringenin Arachis hypogaea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00086
-
 malonyl-CoA P375G mutant enzyme Arachis hypogaea
0.002
-
 malonyl-CoA
-
 Arachis hypogaea
0.016
-
 p-Coumaroyl-CoA P375G mutant enzyme Arachis hypogaea
0.022
-
 p-Coumaroyl-CoA
-
 Arachis hypogaea
0.022
-
 malonyl-CoA malonyl-CoA decarboxylation, P375G mutant enzyme Arachis hypogaea
0.031
-
 malonyl-CoA malonyl-CoA decarboxylation Arachis hypogaea

Organism

Organism UniProt Comment Textmining
Arachis hypogaea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant resveratrol synthase, Ni2+-iminodiacetic acid-Sepharose Arachis hypogaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + malonyl-CoA
-
 Arachis hypogaea 3,4',5-trihydroxystilbene + CoA + CO2 79% resveratrol, side products: 10% p-coumaroyltriacetic acid lactone, 8.6% bisnoryangonin and 1.7% naringenin ?