Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharopolyspora erythraea |
Protein Variants | Comment | Organism |
---|---|---|
C211A | mutation at the ketosynthase domain active site, exhibits no turnover activity, but is still a competent methylmalonyl-ACP decarboxylase | Saccharopolyspora erythraea |
H346A | mutation at the ketosynthase domain, mutant exhibits reduced rates of both chain translocation and chain elongation, with a greater effect on the latter half-reaction | Saccharopolyspora erythraea |
H384A | mutation at the ketosynthase domain, residue contributes to methylmalonyl-ACP decarboxylation | Saccharopolyspora erythraea |
H457A | activity similar to wild-type | Saccharopolyspora erythraea |
K379A | mutation at the ketosynthase domain, residue promotes C-C bond formation | Saccharopolyspora erythraea |
N381A | increase in activity | Saccharopolyspora erythraea |
N455L | activity similar to wild-type | Saccharopolyspora erythraea |
Q386E | about 25% of wild-type activity | Saccharopolyspora erythraea |
S315A | mutation at the ketosynthase domain, residue plays a role in coupling decarboxylation to C-C bond formation | Saccharopolyspora erythraea |
T209A | increase in activity | Saccharopolyspora erythraea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharopolyspora erythraea | Q03131 and Q03132 and Q03133 | Q03131 i.e. modules 1 and 2, Q03132 i.e. modules 3 and 4, Q03133 i.e. modules 5 and 6 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
propanoyl-CoA + (2S)-methylmalonyl-CoA + NADPH | - |
Saccharopolyspora erythraea | (3R,4S,5R,6R)-6-ethyl-4-hydroxy-3,5-dimethyloxan-2-one + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
eryA | - |
Saccharopolyspora erythraea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
propanoyl-CoA | mutant H384A, pH 7.2, temperature not specified in the publication | Saccharopolyspora erythraea | |
0.028 | - |
propanoyl-CoA | mutant Q386E, pH 7.2, temperature not specified in the publication | Saccharopolyspora erythraea | |
0.032 | - |
propanoyl-CoA | mutant H457A, pH 7.2, temperature not specified in the publication | Saccharopolyspora erythraea | |
0.038 | - |
propanoyl-CoA | mutant N381A, pH 7.2, temperature not specified in the publication | Saccharopolyspora erythraea | |
0.038 | - |
propanoyl-CoA | mutant N455L, pH 7.2, temperature not specified in the publication | Saccharopolyspora erythraea | |
0.04 | - |
propanoyl-CoA | mutant S315A, pH 7.2, temperature not specified in the publication | Saccharopolyspora erythraea | |
0.042 | - |
propanoyl-CoA | wild-type, pH 7.2, temperature not specified in the publication | Saccharopolyspora erythraea | |
0.048 | - |
propanoyl-CoA | mutant T209A, pH 7.2, temperature not specified in the publication | Saccharopolyspora erythraea |