Application | Comment | Organism |
---|---|---|
analysis | kinetic assay for the megasynthase that stoichiometrically relates NADPH consumption to polyketide production | Saccharopolyspora erythraea |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharopolyspora erythraea |
Protein Variants | Comment | Organism |
---|---|---|
additional information | development of expression and purification protocols for subunits DEBS2 and DEBS3 from recombinant strains of Escherichia coli | Saccharopolyspora erythraea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharopolyspora erythraea | Q03131 and Q03132 and Q03133 | Q03131, i.e. modules 1 and 2, Q03132, i.e. modules 3 and 4, Q03133, i.e. modules 5 and 6 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
propanoyl-CoA + 5 (2S)-methylmalonyl-CoA + (2S)-ethylmalonyl-CoA + 6 NADPH + 6 H+ | - |
Saccharopolyspora erythraea | (3R,4S,5R,6R)-6-ethyl-4-hydroxy-3,5-dimethyltetrahydro-2H-pyran-2-one + ? | product of truncated enzyme consisting of loading didomain-module1-module2+thioesterase | ? | |
propanoyl-CoA + 5 (2S)-methylmalonyl-CoA + (2S)-ethylmalonyl-CoA + 6 NADPH + 6 H+ | - |
Saccharopolyspora erythraea | (3R,5R,6S)-6-[(2S,3R)-3-hydroxypentan-2-yl]-3,5-dimethyldihydro-2H-pyran-2,4(3H)-dione + ? | product of truncated enzyme consisting of loading didomain-module1-module2-module3+thioesterase | ? | |
propanoyl-CoA + 5 (2S)-methylmalonyl-CoA + (2S)-ethylmalonyl-CoA + 6 NADPH + 6 H+ | - |
Saccharopolyspora erythraea | 8-ethyl-8-desmethyl-6-deoxyerythronolide B + 7 CoA + 6 CO2 + H2O + 6 NADP+ | putative analog, 8-ethyl-8-desmethyl-6-deoxyerythronolide B, is generated by the assembly line in the presence of non-limiting concentrations of ethylmalonyl-CoA and is produced in comparable amounts to the natural 6-dEB product | ? | |
propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH + 6 H+ | - |
Saccharopolyspora erythraea | 6-deoxyerythronolide B + 7 CoA + 6 CO2 + H2O + 6 NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DEBS | - |
Saccharopolyspora erythraea |