Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by dithioerythritol and 2-mercaptoethanol | Raphanus sativus |
General Stability | Organism |
---|---|
1 mg/ml bovine serum albumin stabilizes | Raphanus sativus |
L-malate stabilizes during purification | Raphanus sativus |
thawing after freezing at -20°C leads to 50% loss of activity, bovine serum albumin stabilizes | Raphanus sativus |
Unstable to dilution | Raphanus sativus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-malate | non-competitive | Raphanus sativus | |
L-malate | above 0.05 M | Raphanus sativus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data | Raphanus sativus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for divalent cations | Raphanus sativus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
1 * 51000, SDS-PAGE | Raphanus sativus |
52000 | - |
gel filtration | Raphanus sativus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Raphanus sativus | - |
cv. Saxa Nova | - |
Raphanus sativus | - |
two isoforms | - |
Raphanus sativus | - |
red radish, var. sativus | - |
Purification (Comment) | Organism |
---|---|
2 isozymes on isoelectric focusing of purified enzyme, 4 isoforms on isoelectric focusing of crude extract, in varying proportions depending on light conditions during growth | Raphanus sativus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1-O-sinapoyl-beta-D-glucose + (S)-malate = D-glucose + sinapoyl-(S)-malate | mechanism | Raphanus sativus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Raphanus sativus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.528 | - |
- |
Raphanus sativus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-di-O-sinapoyl-beta-D-glucose + L-malate | at 26% the rate of the reaction with 1-O-sinapoylglucose | Raphanus sativus | ? | - |
? | |
1-O-(4-coumaroyl)-beta-D-glucose + L-malate | poor substrate | Raphanus sativus | D-glucose + 4-coumaroyl-L-malate | - |
? | |
1-O-caffeoyl-beta-D-glucose + L-malate | at 45% the rate of the reaction with 1-O-sinapoylglucose | Raphanus sativus | D-glucose + caffeoyl-L-malate | - |
? | |
1-O-feruloyl-beta-D-glucose + L-malate | at 85% the rate of the reaction with 1-O-sinapoylglucose | Raphanus sativus | D-glucose + feruloyl-L-malate | - |
? | |
1-O-sinapoyl-beta-D-glucose + L-malate | - |
Raphanus sativus | D-glucose + sinapoyl-L-malate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 51000, SDS-PAGE | Raphanus sativus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Raphanus sativus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6.5 | about half-maximal activity at pH 5.5 and 6.5 | Raphanus sativus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
215 | - |
D-malate | - |
Raphanus sativus |