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Literature summary for 2.3.1.90 extracted from

  • Schmidt, S.W.; Denzel, K.; Schilling, G.; Gross, G.G.
    Enzymatic synthesis of 1,6-digalloylglucose from beta-glucogallin by beta-glucogallin:beta-glucogallin 6-0-galloyltransferase from oak leaves (1987), Z. Naturforsch. C, 42, 87-92.
No PubMed abstract available
Search for more literature for 2.3.1.90

General Stability

General Stability Organism
dilution destabilizes Quercus robur
glycerol, 10%, stabilizes Quercus robur

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol above 50 mM Quercus robur
dithioerythritol above 20 mM Quercus robur
additional information no inhibition by metal ions Quercus robur

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information normal Michaelis-Menten kinetics Quercus robur
additional information
-
 additional information substrate saturation not reached at 20 mM Quercus robur

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ion requirement Quercus robur

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
400000
-
 gel filtration Quercus robur

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-O-galloyl-beta-D-glucose + 1-O-galloyl-beta-D-glucose Quercus robur first intermediate in the gallotannin biosynthesis in higher plants, serving as acyl donor and acceptor 1,6-di-O-galloyl-beta-D-glucose + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Quercus robur
-
 oak
-

Purification (Commentary)

Purification (Comment) Organism
partial purification with ammonium sulfate precipitation, DEAE-cellulose chromatography and gel filtration Quercus robur

Source Tissue

Source Tissue Comment Organism Textmining
leaf 2-3 months old Quercus robur
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0743
-
-
 Quercus robur

Storage Stability

Storage Stability Organism
-20°C, stable in the presence of 10% glycerol Quercus robur
2-4°C, t1/2: 2 days, dilute preparation Quercus robur
2-4°C, t1/2: 7 days, concentrated preparation Quercus robur

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-O-galloyl-beta-D-glucose + 1-O-galloyl-beta-D-glucose
-
 Quercus robur 1,6-di-O-galloyl-beta-D-glucose + D-glucose
-
 ?
1-O-galloyl-beta-D-glucose + 1-O-galloyl-beta-D-glucose first intermediate in the gallotannin biosynthesis in higher plants, serving as acyl donor and acceptor Quercus robur 1,6-di-O-galloyl-beta-D-glucose + D-glucose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 25% residual activity at 0°C Quercus robur
30
-
-
 Quercus robur

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 highest stability at pH 4.5-6.5 Quercus robur
55 60 denaturation Quercus robur

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
 Quercus robur

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 about half-maximal activity at pH 5.5 and pH 7.5 Quercus robur

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 6.5 highest stability at 30°C Quercus robur