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Literature summary for 2.3.1.9 extracted from

  • Ithayaraja, M.; Janardan, N.; Wierenga, R.; Savithri, H.; Murthy, M.
    Crystal structure of a thiolase from Escherichia coli at 1.8 A resolution (2016), Acta Crystallogr. F Struct. Biol. Commun., 72, 534-544 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
native enzyme at 1.8 A resolution, in complex with acetyl-CoA, at 1.9 A resolution Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
CoA pH 7.8, 30°C Escherichia coli
0.0176
-
acetoacetyl-CoA pH 7.8, 30°C Escherichia coli
0.1386
-
acetyl-CoA pH 7.8, 30°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
158000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P76461
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 acetyl-CoA
-
Escherichia coli CoA + acetoacetyl-CoA
-
r
CoA + acetoacetyl-CoA
-
Escherichia coli 2 acetyl-CoA
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 40352, calculated from sequence Escherichia coli

Synonyms

Synonyms Comment Organism
AtoB
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5
-
acetyl-CoA pH 7.8, 30°C Escherichia coli
220.5
-
acetoacetyl-CoA pH 7.8, 30°C Escherichia coli