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Literature summary for 2.3.1.9 extracted from

  • Pantazaki, A.; Ioannou, A.; Kyriakidis, D.
    A thermostable beta-ketothiolase of polyhydroxyalkanoates (PHAs) in Thermus thermophilus Purification and biochemical properties (2005), Mol. Cell. Biochem., 269, 27-36 .
    View publication on PubMed
Search for more literature for 2.3.1.9

Activating Compound

Activating Compound Comment Organism Structure
Mg2+ dose-dependent stimulation of the thiolytic reaction Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
acetoacetyl CoA thiolysis reaction shows a substrate inhibition at high concentrations; when one of the substrates (acetoacetyl CoA or CoA) is varied, while the concentrations of the second substrates (CoA or acetoacetyl CoA respectively) remain constant Thermus thermophilus
CoA thiolysis reaction shows a substrate inhibition at high concentrations; when one of the substrates (acetoacetyl CoA or CoA) is varied, while the concentrations of the second substrates (CoA or acetoacetyl CoA respectively) remain constant Thermus thermophilus
N-ethylmaleimide 1 mM Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 CoA pH 8.0, 65°C Thermus thermophilus
0.027
-
 acetoacetyl-CoA pH 8.0, 65°C Thermus thermophilus
0.25
-
 acetyl-CoA pH 8.0, 65°C Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45500
-
 SDS-PAGE Thermus thermophilus
182000
-
 gel filtration Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + acetoacetyl-CoA Thermus thermophilus the enzyme of the biosynthetic pathway for polyhydroxyalkanoates 2 acetyl-CoA
-
 r

Organic Solvent Stability

Organic Solvent Comment Organism
guanidine-HCl denaturation of the enzyme with 6 M guanidine hydrochloride at pH 8.0 and renaturation at pH 7.0 Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus thermophilus

Renatured (Commentary)

Renatured (Comment) Organism
denaturation of the enzyme with 6 M guanidine hydrochloride at pH 8.0 and renaturation at pH 7.0 Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1082
-
 pH 8.0, 65°C, cleavage of acetoacetyl CoA Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 acetyl-CoA
-
 Thermus thermophilus CoA + acetoacetyl-CoA
-
 r
CoA + acetoacetyl-CoA
-
 Thermus thermophilus 2 acetyl-CoA
-
 r
CoA + acetoacetyl-CoA the enzyme of the biosynthetic pathway for polyhydroxyalkanoates Thermus thermophilus 2 acetyl-CoA
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 45500, SDS-PAGE Thermus thermophilus

Synonyms

Synonyms Comment Organism
beta-ketoacyl-CoA thiolase
-
 Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 thiolysis reaction and condensation reaction Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Thermus thermophilus

General Information

General Information Comment Organism
metabolism the enzyme of the biosynthetic pathway for polyhydroxyalkanoates Thermus thermophilus