Application | Comment | Organism |
---|---|---|
biofuel production | the enzyme catalyses the condensation of two acetyl-coenzyme A molecules to form acetoacetyl-CoA in a dedicated pathway towards the biosynthesis of n-butanol, an important solvent and biofuel | Clostridium acetobutylicum |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli B834 cells | Clostridium acetobutylicum |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 100 mM phosphate-citrate pH 4.2, 10% (w/v) polyethylene glycol 3350, 200 mM sodium chloride | Clostridium acetobutylicum |
Protein Variants | Comment | Organism |
---|---|---|
V77Q/N153Y/A286K | the mutant, which is not able to form disulfide bonds, exhibits higher activity than the wild type enzyme | Clostridium acetobutylicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, SDS-PAGE | Clostridium acetobutylicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 acetyl-CoA | Clostridium acetobutylicum | - |
CoA + acetoacetyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium acetobutylicum | P45359 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and HiTrap Q ion exchange column chromatography | Clostridium acetobutylicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 acetyl-CoA | - |
Clostridium acetobutylicum | CoA + acetoacetyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, SDS-PAGE | Clostridium acetobutylicum |
Synonyms | Comment | Organism |
---|---|---|
ThL | - |
Clostridium acetobutylicum |