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Literature summary for 2.3.1.9 extracted from

  • Fujii, T.; Ito, K.; Katsuma, S.; Nakano, R.; Shimada, T.; Ishikawa, Y.
    Molecular and functional characterization of an acetyl-CoA acetyltransferase from the adzuki bean borer moth Ostrinia scapulalis (Lepidoptera: Crambidae) (2009), Insect Biochem. Mol. Biol., 40, 74-78.
    View publication on PubMed
Search for more literature for 2.3.1.9

Application

Application Comment Organism
analysis assay based on the specific absorbance at 303 nm of the Mg2+-enolate complex of acetoacetyl-Coa Ostrinia scapulalis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 cell Ostrinia scapulalis
gene OSAT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of the His-tagged enzyme in Spodoptera frugiperda Sf9 cells using a baculovirus expression system, expression in the cytosol, rather than nucleus and mitochondria Ostrinia scapulalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 41200, calculated, x * 40000, SDS-PAGE Ostrinia scapulalis
41200
-
 x * 41200, calculated, x * 40000, SDS-PAGE Ostrinia scapulalis
42100
-
 x * 42100 Ostrinia scapulalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 acetyl-CoA Ostrinia scapulalis the thiolase is involved in the synthesis and catabolism of fatty acids acetoacetyl-CoA + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Ostrinia scapulalis B7XEI5
-
-
Ostrinia scapulalis B7XEI5 adzuki bean borer moth, female moths, gene Osat1
-

Source Tissue

Source Tissue Comment Organism Textmining
egg
-
 Ostrinia scapulalis
-
epidermis
-
 Ostrinia scapulalis
-
fat body
-
 Ostrinia scapulalis
-
flight muscle
-
 Ostrinia scapulalis
-
leg
-
 Ostrinia scapulalis
-
additional information Osat1 is expressed in all adult tissues Ostrinia scapulalis
-
additional information mRNA is ubiquitously expressed in 6 adult tissues including pheromone gland Ostrinia scapulalis
-
pheromone gland
-
 Ostrinia scapulalis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 acetyl-CoA
-
 Ostrinia scapulalis acetoacetyl-CoA + CoA
-
 ?
2 acetyl-CoA the thiolase is involved in the synthesis and catabolism of fatty acids Ostrinia scapulalis acetoacetyl-CoA + CoA
-
 ?

Subunits

Subunits Comment Organism
? x * 42100 Ostrinia scapulalis
? x * 41200, calculated, x * 40000, SDS-PAGE Ostrinia scapulalis

Synonyms

Synonyms Comment Organism
acetyl-CoA acetyltransferase
-
 Ostrinia scapulalis
OsAT1
-
 Ostrinia scapulalis