Cloned (Comment) | Organism |
---|---|
- |
Zoogloea ramigera |
Crystallization (Comment) | Organism |
---|---|
wild-type thiolase and acetylated thiolase complexed with CoA, C89A mutant thiolase complexed with acetyl-CoA and acetoacetyl-CoA, Q64A mutant thiolase | Zoogloea ramigera |
Protein Variants | Comment | Organism |
---|---|---|
C89A | no thiolytic activity towards acetoacetyl-CoA | Zoogloea ramigera |
Q64A | 30% lower kcat than that of the wild-type | Zoogloea ramigera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + acetoacetyl-CoA | Zoogloea ramigera | - |
acetyl-CoA + acetyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zoogloea ramigera | P07097 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 acetyl-CoA = CoA + acetoacetyl-CoA | reaction involves 2 chemically distinct steps: acetyl group transfer from acetyl-CoA to Cys89, and transfer of this acetyl group to a second acetyl-CoA in the Claisen condensation step to form acetoacetyl-CoA | Zoogloea ramigera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + acetoacetyl-CoA | - |
Zoogloea ramigera | acetyl-CoA + acetyl-CoA | - |
? | |
CoA + acetoacetyl-CoA | - |
Zoogloea ramigera | acetyl-CoA + acetyl-CoA | - |
r |