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Literature summary for 2.3.1.9 extracted from
- The kinetic mechanism and properties of the cytoplasmic acetoacetyl-coenzyme A thiolase from rat liver (1974), Biochem. J., 139, 109-121.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CoA | competitive vs. acetoacetyl-CoA in thiolysis | Rattus norvegicus |  |
| iodoacetamide | 0.005 mM, inactivation half-life: 3 min | Rattus norvegicus | |
| Mg2+ | inhibits the rate of acetoacetyl-CoA thiolysis but not the rate of synthesis of acetoacetyl-CoA | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
4 * 40000, enzyme A and B, SDS-PAGE | Rattus norvegicus |
| 170000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + acetoacetyl-CoA | Rattus norvegicus | - |
acetyl-CoA + acetyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| pH 5.5, DEAE-cellulose, calcium phosphate column, celulose phosphate column | Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2 acetyl-CoA = CoA + acetoacetyl-CoA | mechanism | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + acetoacetyl-CoA | - |
Rattus norvegicus | acetyl-CoA + acetyl-CoA | - |
? | |
| CoA + acetoacetyl-CoA | thiolysis is strongly preferred | Rattus norvegicus | acetyl-CoA + acetyl-CoA | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 40000, enzyme A and B, SDS-PAGE | Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.067 | - |
CoA | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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