Cloned (Comment) | Organism |
---|---|
expression of AANAT-(2207) and mutant enzymes in Escherichia coli strain BL21(DE3) | Ovis aries |
Protein Variants | Comment | Organism |
---|---|---|
I57A/V59A | site-directed mutagenesis, the mutant shows reduced enzyme activity and altered inhibitor binding compared to the wild-type enzyme | Ovis aries |
additional information | construction of truncation mutants with reduced enzyme activity | Ovis aries |
P64A | site-directed mutagenesis, the mutant shows reduced enzyme activity and altered inhibitor binding compared to the wild-type enzyme | Ovis aries |
P64G | site-directed mutagenesis, the mutant shows reduced enzyme activity and altered inhibitor binding compared to the wild-type enzyme | Ovis aries |
P64W | site-directed mutagenesis, the mutant shows reduced enzyme activity and altered inhibitor binding compared to the wild-type enzyme | Ovis aries |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoA-S-N-acetyl-7-hydroxynaphthylethylamine | fluorescent variant of CoA-T, a bisubstrate inhibitor, synthesis and enzyme binding structure, molecular modelling, overview | Ovis aries | |
tryptamine-coenzyme A | a bisubstrate inhibitor, enzyme binding structure, molecular modelling, overview | Ovis aries |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of recombinant wild-type and mutant enzymes, substrate tryptamine, overview | Ovis aries | |
0.33 | - |
tryptamine | pH 6.8, 37°C, recombinant wild-type enzyme | Ovis aries | |
2.3 | - |
tryptamine | pH 6.8, 37°C, recombinant mutant I57A/V59A | Ovis aries | |
4 | - |
tryptamine | above, pH 6.8, 37°C, recombinant mutants P64A, P64G, P64W, and recombinant truncation mutants | Ovis aries |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + serotonin | Ovis aries | penultimate step in melatonin biosynthesis | CoA + N-acetylserotonin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ovis aries | Q29495 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pineal gland | - |
Ovis aries | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme activities of recombinant wild-type and mutant enzymes, overview | Ovis aries |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + serotonin | penultimate step in melatonin biosynthesis | Ovis aries | CoA + N-acetylserotonin | - |
? | |
acetyl-CoA + serotonin | reaction mechanism involving Pro64 that plays a critical role in structure and catalysis | Ovis aries | CoA + N-acetylserotonin | - |
? | |
acetyl-CoA + tryptamine | - |
Ovis aries | CoA + N-acetyltryptamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AANAT | - |
Ovis aries |
More | AANAT is a member of the GCN5-related N-acetyltransferase, GNAT, superfamily of acetyltransferases | Ovis aries |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Ovis aries |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Ovis aries |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00015 | - |
tryptamine-CoA | - |
Ovis aries | |
0.0003 | - |
CoA-S-N-acetyl-7-hydroxynaphthylethylamine | - |
Ovis aries |