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Literature summary for 2.3.1.87 extracted from

  • Pavlicek, J.; Coon, S.L.; Ganguly, S.; Weller, J.L.; Hassan, S.A.; Sackett, D.L.; Klein, D.C.
    Evidence that proline focuses movement of the floppy loop of arylalkylamine N-acetyltransferase (EC 2.3.1.87) (2008), J. Biol. Chem., 283, 14552-14558.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of AANAT-(2–207) and mutant enzymes in Escherichia coli strain BL21(DE3) Ovis aries

Protein Variants

Protein Variants Comment Organism
I57A/V59A site-directed mutagenesis, the mutant shows reduced enzyme activity and altered inhibitor binding compared to the wild-type enzyme Ovis aries
additional information construction of truncation mutants with reduced enzyme activity Ovis aries
P64A site-directed mutagenesis, the mutant shows reduced enzyme activity and altered inhibitor binding compared to the wild-type enzyme Ovis aries
P64G site-directed mutagenesis, the mutant shows reduced enzyme activity and altered inhibitor binding compared to the wild-type enzyme Ovis aries
P64W site-directed mutagenesis, the mutant shows reduced enzyme activity and altered inhibitor binding compared to the wild-type enzyme Ovis aries

Inhibitors

Inhibitors Comment Organism Structure
CoA-S-N-acetyl-7-hydroxynaphthylethylamine fluorescent variant of CoA-T, a bisubstrate inhibitor, synthesis and enzyme binding structure, molecular modelling, overview Ovis aries
tryptamine-coenzyme A a bisubstrate inhibitor, enzyme binding structure, molecular modelling, overview Ovis aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of recombinant wild-type and mutant enzymes, substrate tryptamine, overview Ovis aries
0.33
-
tryptamine pH 6.8, 37°C, recombinant wild-type enzyme Ovis aries
2.3
-
tryptamine pH 6.8, 37°C, recombinant mutant I57A/V59A Ovis aries
4
-
tryptamine above, pH 6.8, 37°C, recombinant mutants P64A, P64G, P64W, and recombinant truncation mutants Ovis aries

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + serotonin Ovis aries penultimate step in melatonin biosynthesis CoA + N-acetylserotonin
-
?

Organism

Organism UniProt Comment Textmining
Ovis aries Q29495
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pineal gland
-
Ovis aries
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
enzyme activities of recombinant wild-type and mutant enzymes, overview Ovis aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + serotonin penultimate step in melatonin biosynthesis Ovis aries CoA + N-acetylserotonin
-
?
acetyl-CoA + serotonin reaction mechanism involving Pro64 that plays a critical role in structure and catalysis Ovis aries CoA + N-acetylserotonin
-
?
acetyl-CoA + tryptamine
-
Ovis aries CoA + N-acetyltryptamine
-
?

Synonyms

Synonyms Comment Organism
AANAT
-
Ovis aries
More AANAT is a member of the GCN5-related N-acetyltransferase, GNAT, superfamily of acetyltransferases Ovis aries

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Ovis aries

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Ovis aries

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00015
-
tryptamine-CoA
-
Ovis aries
0.0003
-
CoA-S-N-acetyl-7-hydroxynaphthylethylamine
-
Ovis aries