Any feedback?
Literature summary for 2.3.1.86 extracted from
- De novo biosynthesis of 8-hydroxyoctanoic acid via a medium-chain length specific fatty acid synthase and cytochrome P450 in Saccharomyces cerevisiae (2020), Metab. Eng. Commun., 10, e00111 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | expression of heterologous cytochrome P450 enzyme in combination with their cognate reductase for omega-hydroxylation of octanoic acid in a yeast strain, whose fatty acid synthase is engineered for octanoic acid production, results in de novo biosynthesis of 8-hydroxyoctanoic acid up to 3 mg/l. Cytochromes P450 activities are limiting 8-hydroxyoctanoic acid synthesis. The hydroxylation of both externally added and intracellularly produced octanoic acid is strongly dependent on the carbon source used, with ethanol being preferred | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R1834K | mutation enables production of C8 faty acids with high specificity | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
