Application | Comment | Organism |
---|---|---|
synthesis | short-chain acyl-CoA producing yeast Fas1 mutant R1834K/Fas2 fatty acid synthase variant is expressed together with carboxylic acid reductase from Mycobacterium marinum and phosphopantetheinyl transferase Sfp from Bacillus subtilis in a Saccharomyces cerevisiae DELTAfas1 DELTAfas2 DELTAfaa2 mutant strain. The synthesized octanoyl-CoA is endogenously converted to 1-octanol up to a titer of 26.0 mg/l in a 72-h fermentation. When octanoic acid is supplied externally to the yeast cells, it can be efficiently converted to 1-octanol. Additional overexpression of aldehyde reductase Ahr from Escherichia coli nearly completely prevents accumulation of octanoic acid and increases 1-octanol titers up to 49.5 mg/l. 1-octanol acts inhibitive before secretion | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
R1834K | mutation in Fas1 gene. Mutant produces short-chain acyl-CoAs | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |