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Literature summary for 2.3.1.86 extracted from

  • Stoops, J.K.; Wakil, S.J.
    Studies on the yeast fatty acid synthetase. Subunit composition and structural organization of a large multifunctional enzyme complex (1978), J. Biol. Chem., 253, 4464-4475.
    View publication on PubMed
Search for more literature for 2.3.1.86

General Stability

General Stability Organism
PMSF ensures isolation of native enzyme Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
methylamine tungstad inactivation within 24 h Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid composition Saccharomyces cerevisiae
additional information
-
 alpha6beta6-complex of multifunctional subunits Saccharomyces cerevisiae
203000
-
 alpha6,beta6, 6 * 213000 + 6 * 203000, Tris-glycine-SDS-PAGE Saccharomyces cerevisiae
213000
-
 alpha6,beta6, 6 * 213000 + 6 * 203000, Tris-glycine-SDS-PAGE Saccharomyces cerevisiae
2370000
-
 sedimentation equilibrium method Saccharomyces cerevisiae
2400000
-
 sedimentation velocity Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + malonyl-CoA + NADH + NADPH Saccharomyces cerevisiae multifunctional enzyme involved in yeast fat metabolism ?
-
 ?
acetyl-CoA + malonyl-CoA + NADH + NADPH Saccharomyces cerevisiae Fleishmann multifunctional enzyme involved in yeast fat metabolism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 wild type and protease-negative pep4-mutant
-
Saccharomyces cerevisiae Fleishmann
-
 wild type and protease-negative pep4-mutant
-

Purification (Commentary)

Purification (Comment) Organism
isolation of peptides after proteolysis Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ = hexadecanoyl-CoA + 7 CoA + 7 CO2 + 14 NADP+ + 7 H2O structure Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5 4
-
 Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-20°C, 1 month Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + malonyl-CoA + NADH + NADPH multifunctional enzyme involved in yeast fat metabolism Saccharomyces cerevisiae ?
-
 ?
acetyl-CoA + malonyl-CoA + NADH + NADPH multifunctional enzyme involved in yeast fat metabolism Saccharomyces cerevisiae Fleishmann ?
-
 ?

Subunits

Subunits Comment Organism
dodecamer alpha6,beta6, 6 * 213000 + 6 * 203000, Tris-glycine-SDS-PAGE Saccharomyces cerevisiae
dodecamer active enzyme centrifugation Saccharomyces cerevisiae
More
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
4'-phosphopantetheine 4.0-5.0 mol per mol enzyme or about 1 mol per 2 subunits Saccharomyces cerevisiae
NADPH requirement Saccharomyces cerevisiae