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Literature summary for 2.3.1.86 extracted from
- Fatty acid synthesis and its regulation (1983), Annu. Rev. Biochem., 52, 537-579.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| transformation technique, plasmid YEpFAS2 transformed to and expressed in Escherichia coli maxi-cells | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,3-Dibromo-2-propanone | - |
Saccharomyces cerevisiae |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mycolicibacterium smegmatis |
| additional information | - |
alpha6beta6-complex of multifunctional subunits | Saccharomyces cerevisiae |
| additional information | - |
amino acid sequence of acyl-carrier-protein | Saccharomyces cerevisiae |
| 180000 | - |
alpha6,beta6, 6 * 185000 + 6 * 180000, SDS-PAGE | Saccharomyces cerevisiae |
| 185000 | - |
alpha6,beta6, 6 * 185000 + 6 * 180000, SDS-PAGE | Saccharomyces cerevisiae |
| 203000 | - |
alpha6,beta6, 6 * 213000 + 6 * 203000, Tris-glycine-SDS-PAGE | Saccharomyces cerevisiae |
| 213000 | - |
alpha6,beta6, 6 * 213000 + 6 * 203000, Tris-glycine-SDS-PAGE | Saccharomyces cerevisiae |
| 2400000 | - |
sedimentation velocity | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + malonyl-CoA + NADH + NADPH | Saccharomyces cerevisiae | multifunctional enzyme involved in yeast fat metabolism | ? | - |
? | |
| acetyl-CoA + malonyl-CoA + NADH + NADPH | Mycolicibacterium smegmatis | multifunctional enzyme involved in yeast fat metabolism | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isolation of alpha- and beta-subunits | Saccharomyces cerevisiae |
| isolation of alpha- and beta-subunits by citraconic and dimethylmaleic anhydride modification | Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ = hexadecanoyl-CoA + 7 CoA + 7 CO2 + 14 NADP+ + 7 H2O | reaction mechanism | Saccharomyces cerevisiae | |
| acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ = hexadecanoyl-CoA + 7 CoA + 7 CO2 + 14 NADP+ + 7 H2O | reaction mechanism | Mycolicibacterium smegmatis | |
| acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ = hexadecanoyl-CoA + 7 CoA + 7 CO2 + 14 NADP+ + 7 H2O | structure | Saccharomyces cerevisiae | |
| acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ = hexadecanoyl-CoA + 7 CoA + 7 CO2 + 14 NADP+ + 7 H2O | structure and regulation | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + 7 malonyl-CoA + 7 NADH + 7 NADPH + 14 H+ | - |
Mycolicibacterium smegmatis | palmitoyl-CoA + 7 CoA + 7 CO2 + 7 NAD+ + 7 NADP+ + 7 H2O | - |
? | |
| acetyl-CoA + 7 malonyl-CoA + 7 NADH + 7 NADPH + 14 H+ | stable multifunctional enzyme complex: carries acetyl-CoA and malonyl-CoA transacylase, beta-ketoacyl reductase, beta-hydroxyacyl dehydrase activities on beta-subunits and condensing enzyme, i.e. beta-ketoacyl synthetase, enoylacyl reductase activities and acyl-carrier-protein components on alpha-subunits | Saccharomyces cerevisiae | palmitoyl-CoA + 7 CoA + 7 CO2 + 7 NAD+ + 7 NADP+ + 7 H2O | - |
? | |
| acetyl-CoA + malonyl-CoA + NADH + NADPH | multifunctional enzyme involved in yeast fat metabolism | Saccharomyces cerevisiae | ? | - |
? | |
| acetyl-CoA + malonyl-CoA + NADH + NADPH | multifunctional enzyme involved in yeast fat metabolism | Mycolicibacterium smegmatis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dodecamer | alpha6,beta6, 6 * 185000 + 6 * 180000, SDS-PAGE | Saccharomyces cerevisiae |
| dodecamer | alpha6,beta6, 6 * 213000 + 6 * 203000, Tris-glycine-SDS-PAGE | Saccharomyces cerevisiae |
| More | - |
Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 4'-phosphopantetheine | 4.0-5.0 mol per mol enzyme or about 1 mol per 2 subunits | Saccharomyces cerevisiae | |
| 4'-phosphopantetheine | 4.0-5.0 mol per mol enzyme | Saccharomyces cerevisiae | |
| 4'-phosphopantetheine | alpha-subunit bears prosthetic group | Saccharomyces cerevisiae | |
| FMN | requirement, enoyl reductase activity, associated with beta-subunit, 6 mol per mol synthetase | Saccharomyces cerevisiae | |
| NADPH | requirement | Saccharomyces cerevisiae | |
| NADPH | requirement | Mycolicibacterium smegmatis | |
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