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Literature summary for 2.3.1.85 extracted from

  • Rittner, A.; Paithankar, K.S.; Drexler, D.J.; Himmler, A.; Grininger, M.
    Probing the modularity of megasynthases by rational engineering of a fatty acid synthase Type I (2019), Protein Sci., 28, 414-428 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.85

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information dissection of the enzyme into condensing, processing and terminating parts, engineering and reassembly to generate new polyketide synthase-like modules as well as bimodular constructs. The reengineered modules resemble all four common types of polyketide synthases Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus P19096
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-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific malonyl consumption rate is 282 nmol per min per mg protein, pH 7.0, 25°C Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoacetyl-CoA + n malonyl-CoA + 2n NADPH + 2n H+
-
 Mus musculus a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP+
-
 ?
acetyl-CoA + n malonyl-CoA + 2n NADPH + 2n H+
-
 Mus musculus a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP+
-
 ?
butanoyl-CoA + n malonyl-CoA + 2n NADPH + 2n H+
-
 Mus musculus a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP+
-
 ?
additional information under non-reducing conditions in the absence of NADPH, FASN produces triacetic acid lactone with an averaged malonyl consumption rate of 46 nmol per min per mg protein Mus musculus ?
-
-
octanoyl-CoA + n malonyl-CoA + 2n NADPH + 2n H+
-
 Mus musculus a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP+
-
 ?